Description

Pst I CAS NO 81295-32-1 is a high-purity restriction enzyme, a critical tool for molecular biology research and biotechnological applications. This endonuclease is essential for precise DNA manipulation, enabling accurate genetic analysis and recombinant DNA technology. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic kit manufacturers for applications including gene cloning, genotyping, and DNA mapping.

Application

• Molecular Cloning: Essential for cutting DNA at specific recognition sites to insert genes into vectors.
• Genotyping and DNA Analysis: Used in Restriction Fragment Length Polymorphism (RFLP) analysis for genetic fingerprinting and mutation detection.
• Recombinant DNA Technology: A fundamental reagent for constructing plasmids and other recombinant molecules in biopharmaceutical development.
• Diagnostic Assay Development: Incorporated into kits for the detection of specific genetic markers or pathogens.
• Genome Mapping: Employed to digest genomic DNA for physical mapping and sequencing projects.
• Quality Control in Biomanufacturing: Used to verify plasmid identity and integrity during upstream process development.

Basic Information

Product Name	Pst I
CAS No.	81295-32-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PstI; PstI Restriction Endonuclease; PstI Enzyme; Endodeoxyribonuclease PstI; Restriction Enzyme PstI; Type II Restriction Enzyme PstI; CTGCAG-specific endonuclease
EINECS	Contact for details

Quality Control

Our Pst I is manufactured under stringent conditions to ensure high specific activity and exceptional purity, free from contaminating nucleases and phosphatases. Each lot undergoes rigorous functional testing, including unit definition assays and specificity verification. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and stability is provided with every shipment to guarantee batch-to-batch consistency for your critical research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. The product is supplied in a buffered glycerol solution. Avoid repeated freeze-thaw cycles. Handle on ice when in use. Keep the container tightly sealed to prevent moisture absorption and contamination.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cuts pBR322 DNA at the known single site
Specific Activity	≥ 20,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Exonuclease Activity	Not detectable
Endonuclease Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, pH 7.4, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.