Description

Kpn I is a high-purity restriction endonuclease enzyme, a critical tool for precise DNA manipulation in molecular biology research and biotechnological applications. Its primary value lies in its ability to recognize and cleave specific DNA sequences with high fidelity, enabling recombinant DNA technology, gene cloning, and genomic analysis. This product is essential for researchers and process developers in the pharmaceutical, biotechnology, and academic sectors who require reliable, activity-guaranteed enzymes for genetic engineering workflows. Consistent quality and batch-to-batch reproducibility are paramount for successful experimental outcomes.

Application

• Recombinant DNA Technology: For the precise cutting and insertion of DNA fragments into plasmid vectors.
• Gene Cloning and Mapping: Essential for constructing genomic libraries and performing restriction fragment length polymorphism (RFLP) analysis.
• Molecular Diagnostics: Used in techniques for genetic fingerprinting and the detection of specific gene sequences.
• Biopharmaceutical Development: A key reagent in the research and development phase of gene therapies and plasmid DNA production.
• Academic and Industrial Research: Fundamental for basic genetic research, synthetic biology, and metabolic engineering projects.
• Quality Control in Biotechnology: Employed to verify plasmid identity and integrity through restriction digest analysis.

Basic Information

Product Name	Kpn I
CAS No.	81295-27-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	KpnI; Endodeoxyribonuclease KpnI; Restriction enzyme Kpn I; Type II restriction enzyme KpnI; Klebsiella pneumoniae restriction endonuclease I; Enzyme Commission Number 3.1.21.4
EINECS	Contact for details

Quality Control

Our Kpn I is produced and tested under stringent quality management systems to ensure the highest standards of purity and specific activity. Each batch is subjected to rigorous analytical testing, including assays for endonuclease activity, absence of non-specific nuclease activity (exonuclease and phosphatase), and purity assessment via SDS-PAGE. A comprehensive Certificate of Analysis (COA) detailing purity, activity units, and buffer composition is provided with every shipment to guarantee performance and traceability for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. Avoid repeated freeze-thaw cycles to maintain optimal enzymatic activity. The product should be handled on ice during use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves GGTACC sequence specifically
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Exonuclease Activity	Not detectable
Phosphatase Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.