Description

Bcl I CAS NO 81295-11-6 is a high-purity chemical compound, specifically a restriction enzyme, widely utilized in molecular biology research and biotechnology applications. This product is essential for precise DNA cleavage at specific recognition sites, enabling critical workflows in genetic engineering and genomic analysis. It is a fundamental reagent for pharmaceutical R&D, academic research institutions, and diagnostic kit manufacturers requiring reliable and consistent enzymatic activity for reproducible results.

Application

• Molecular Cloning: Essential for the digestion and manipulation of DNA fragments in plasmid construction and recombinant DNA technology.
• Genotyping and DNA Mapping: Used in restriction fragment length polymorphism (RFLP) analysis and genome mapping studies.
• Diagnostic Assay Development: A key component in the development of molecular diagnostic tests and kits for genetic disorders and pathogen detection.
• Biopharmaceutical Research: Employed in the research and development of gene therapies, vaccines, and other biologics.
• Forensic Analysis: Applied in DNA fingerprinting and forensic science for sample identification and analysis.
• Academic & Industrial R&D: A standard tool in life science laboratories for fundamental research in genetics, cell biology, and biochemistry.

Basic Information

Product Name	Bcl I
CAS No.	81295-11-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	BclI; BclI Restriction Enzyme; Endodeoxyribonuclease BclI; Restriction Endonuclease BclI; Bcl I (Bacillus caldolyticus); BclI-HF; BclI restriction endonuclease
EINECS	Contact for details

Quality Control

Our Bcl I enzyme is produced and tested under stringent quality management systems to ensure the highest standards of purity, specificity, and activity. Each lot undergoes rigorous analytical testing, including activity assays, purity assessment via SDS-PAGE, and tests for contaminating nuclease activities. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and storage buffer composition is provided with every shipment to guarantee batch-to-batch consistency and performance for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. The product is hygroscopic (moisture-sensitive) and should be handled under appropriate conditions to prevent activity loss. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves DNA at the specific recognition sequence (T/GATCA)
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Contaminating Nuclease Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 µg/ml BSA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.