Description

n-Formylnorleucyl-Leucyl-Phenylalanine Chloromethyl Ketone is a potent and irreversible inhibitor of serine proteases, specifically designed to target enzymes like chymotrypsin and cathepsin G. This compound matters as a critical research tool for studying protease function, signaling pathways, and inflammatory responses in biological systems. It is primarily needed by pharmaceutical R&D teams, academic biochemistry laboratories, and biotechnology companies focused on developing novel therapeutic agents for conditions involving dysregulated protease activity.

Application

• Protease Inhibition Studies: Used as a specific active-site directed inhibitor for mechanistic studies of serine proteases, particularly chymotrypsin-like enzymes.
• Biochemical Research: A key reagent for investigating the role of proteases in cellular processes, including apoptosis, inflammation, and protein degradation.
• Pharmaceutical Development: Serves as a lead compound or a pharmacological tool in the discovery and validation of new anti-inflammatory or anti-cancer drug targets.
• Enzyme Characterization: Employed to characterize enzyme kinetics, active site topology, and inhibitor binding affinities in vitro.
• Cell Signaling Pathway Analysis: Utilized to selectively block specific protease activity to elucidate its function within complex signaling networks.

Basic Information

Product Name	n-Formylnorleucyl-Leucyl-Phenylalanine Chloromethyl Ketone
CAS No.	81134-56-7
Molecular Formula	C27H42ClN3O5
Molecular Weight	524.09 g/mol
Synonyms	N-Formyl-Nle-Leu-Phe-CH2Cl; Z-Gly-Leu-Phe-chloromethyl ketone (related analog); Protease Inhibitor 81134-56-7; Chymotrypsin Inhibitor; Cathepsin G Inhibitor; N-Formylnorleucine-Leucine-Phenylalanine Chloromethyl Ketone; FNLF-CMK; N-Formyl-Nle-Leu-Phe-CMK
EINECS	Contact for details

Quality Control

Our n-Formylnorleucyl-Leucyl-Phenylalanine Chloromethyl Ketone is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and structural confirmation by NMR and mass spectrometry, to ensure it meets the high standards required for research applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its sensitivity to moisture and oxidation. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and exposure to humidity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.