Description

5-Fluorouracil n-β-D-Glucuronide Methyl Ester, 2,3,4-Triacetate is a key protected intermediate in the synthesis of glucuronidated metabolites of the chemotherapeutic agent 5-fluorouracil. This compound matters for enabling precise pharmacokinetic and metabolic studies, as it serves as a crucial precursor for generating analytical standards and labeled tracers. Pharmaceutical researchers and diagnostic developers need this high-purity intermediate to investigate drug metabolism pathways, develop bioanalytical assays, and support regulatory submissions for oncology treatments.

Application

• Metabolite Reference Standard Synthesis: Primary use as a protected synthetic intermediate for preparing the active glucuronide metabolite of 5-fluorouracil for use as an analytical standard.
• Pharmacokinetic & ADME Studies: Enables the study of absorption, distribution, metabolism, and excretion (ADME) of 5-fluorouracil in preclinical and clinical research.
• Diagnostic Kit Development: Serves as a critical raw material in the development of enzyme-linked immunosorbent assays (ELISAs) and other diagnostic kits for monitoring 5-FU levels in patients.
• Isotope-Labeled Tracer Production: Used as a starting point for synthesizing stable isotope-labeled (e.g., 13C, 15N) versions of the metabolite for mass spectrometry-based quantification.
• Drug Metabolism Research: Facilitates investigations into the role of glucuronidation in the activation and deactivation pathways of fluoropyrimidine drugs.
• Bioconjugation & Prodrug Design: Acts as a specialized building block in the design of novel prodrugs or targeted conjugates aimed at improving therapeutic efficacy and reducing side effects.

Basic Information

Product Name	5-Fluorouracil n-β-D-Glucuronide Methyl Ester, 2,3,4-Triacetate
CAS No.	77476-81-4
Molecular Formula	C₂₀H₂₃FN₂O₁₂
Molecular Weight	502.40 g/mol
Synonyms	5-FU Glucuronide Methyl Ester Triacetate; 1-((2R,3S,4S,5R,6S)-3,4,5-Triacetoxy-6-(methoxycarbonyl)tetrahydro-2H-pyran-2-yl)-5-fluoro-1,2,3,4-tetrahydropyrimidine-2,4-dione; Methyl (2R,3S,4S,5R,6S)-3,4,5-triacetoxy-6-(5-fluoro-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydro-2H-pyran-2-carboxylate; 5-Fluorouracil 1-β-D-Glucuronide Methyl Ester Triacetate; 5-FU-β-Glucuronide Methyl Ester Triacetate; 1-(Methyl 2,3,4-tri-O-acetyl-β-D-glucopyranuronosyl)-5-fluorouracil; 5-Fluorouridine-1-β-D-glucuronide methyl ester triacetate (common misnomer)
EINECS	Contact for details

Quality Control

Our 5-Fluorouracil n-β-D-Glucuronide Methyl Ester, 2,3,4-Triacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry, to ensure it meets the exacting standards required for pharmaceutical research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, impurities, and analytical methods is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.