Description

Tyrosyl-Alanyl-Glycyl-Phenylalanyl-Leucine Chloromethyl Ketone is a synthetic peptide-based chloromethyl ketone, a potent and irreversible active-site directed inhibitor of serine proteases. This compound is critical for biochemical research and pharmaceutical development, enabling precise study and modulation of proteolytic enzyme activity. It is primarily utilized by research institutions, diagnostic manufacturers, and pharmaceutical companies engaged in protease inhibitor discovery and mechanistic enzymology studies.

Application

• Biochemical Research: As a specific, irreversible inhibitor for studying the structure, function, and mechanism of chymotrypsin-like serine proteases.
• Protease Inhibitor Development: Serves as a key pharmacophore and lead compound in the design of therapeutic protease inhibitors for conditions involving dysregulated proteolysis.
• Enzymology Studies: Used to titrate active enzyme concentrations and to investigate enzyme kinetics and inhibition pathways in vitro.
• Diagnostic Reagent Manufacturing: Incorporated into diagnostic kits and assays that require specific protease inhibition for accurate measurement of other analytes.
• Cell Biology & Physiology: Applied in cellular models to probe the role of specific serine proteases in signaling pathways and physiological processes.
• Pharmaceutical Reference Standard: Employed as a high-purity reference material for quality control and method development in drug discovery pipelines.

Basic Information

Product Name	Tyrosyl-Alanyl-Glycyl-Phenylalanyl-Leucine Chloromethyl Ketone
CAS No.	76960-26-4
Molecular Formula	C34H46ClN5O7
Molecular Weight	672.21 g/mol
Synonyms	YAGFL-CMK; Tyr-Ala-Gly-Phe-Leu Chloromethyl Ketone; TPCK-related Peptide Inhibitor; Chymotrypsin Substrate Analog Inhibitor; N-α-Carbobenzoxy-Tyr-Ala-Gly-Phe-Leu Chloromethyl Ketone; Z-Tyr-Ala-Gly-Phe-Leu-CMK; Protease Inhibitor 76960-26-4; Acetyl-Tyr-Ala-Gly-Phe-Leu Chloromethyl Ketone (variants)
EINECS	Contact for details

Quality Control

Our Tyrosyl-Alanyl-Glycyl-Phenylalanyl-Leucine Chloromethyl Ketone is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards, and custom synthesis to meet specific purity or packaging requirements is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.