Description

E-64C CAS NO 76684-89-4 is a potent, cell-permeable, and irreversible cysteine protease inhibitor. This compound is critical for researchers and manufacturers requiring precise control over proteolytic activity in biological systems. It is essential for the pharmaceutical, biotechnology, and life science research industries, particularly in the study of apoptosis, protein degradation pathways, and the development of therapeutic agents.

Application

• Biochemical Research: A fundamental tool for inhibiting cysteine proteases like calpains, cathepsins, and papain in in vitro and cell-based assays.
• Pharmaceutical Development: Used in target validation and mechanistic studies for diseases involving dysregulated protease activity, such as muscular dystrophy, cancer, and neurodegenerative disorders.
• Cell Biology Studies: Employed to investigate the role of cysteine proteases in critical cellular processes including apoptosis (programmed cell death), autophagy, and signal transduction.
• Protein Chemistry: Serves as a protective agent during protein extraction and purification to prevent unwanted degradation by cysteine proteases.
• Diagnostic Reagent Manufacturing: A key component in the formulation of specific diagnostic kits and research reagents designed to measure protease activity or inhibition.

Basic Information

Product Name	E-64C
CAS No.	76684-89-4
Molecular Formula	C₁₅H₂₇N₃O₅
Molecular Weight	329.40 g/mol
Synonyms	Loxistatin; Loxistatin (E64c); (2S,3S)-3-[[(2S)-3-Methyl-1-oxo-1-[(phenylmethoxy)amino]butan-2-yl]carbamoyl]oxirane-2-carboxylic Acid; E-64c; Epoxysuccinyl-L-leucylamido-3-methylbutane; N-(N-(L-3-trans-Carboxyoxiran-2-carbonyl)-L-leucyl)-agmatine; Protease Inhibitor E64c; EST
EINECS	Contact for details

Quality Control

Our E-64C is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture content. We provide full traceability and Certificates of Analysis (COA) are available for every shipment, ensuring compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.