Description

Acetylphenyl-Alanyl-Lysine Chloromethyl Ketone is a specialized peptide-based chloromethyl ketone inhibitor, a key tool compound for biochemical and pharmacological research. Its primary value lies in its potent and selective inhibition of serine proteases, enabling precise study of enzymatic pathways and mechanisms. This compound is essential for researchers and developers in the pharmaceutical and life sciences sectors, particularly those focused on protease-targeted drug discovery, enzyme kinetics, and the development of novel therapeutic agents.

Application

• Protease Inhibition Research: Serves as a potent and selective active-site directed inhibitor for studying serine protease function and mechanism.
• Drug Discovery & Development: Used as a key pharmacophore and lead compound in the design of novel protease-targeted therapeutics.
• Biochemical Assay Development: Employed in the development and validation of high-throughput screening assays for protease activity.
• Enzyme Kinetics Studies: A critical tool for determining kinetic parameters (e.g., Ki, kinact) and understanding catalytic mechanisms.
• Apoptosis & Cell Signaling Research: Applied in studies investigating the role of specific proteases in programmed cell death and signal transduction pathways.
• Structural Biology: Used to create stable enzyme-inhibitor complexes for X-ray crystallography and other structural analysis techniques.

Basic Information

Product Name	Acetylphenyl-Alanyl-Lysine Chloromethyl Ketone
CAS No.	76157-63-6
Molecular Formula	C22H32ClN4O5
Molecular Weight	467.97 g/mol
Synonyms	Ac-Phe-Ala-Lys-CH2Cl; N-Acetyl-Phe-Ala-Lys Chloromethyl Ketone; Acetyl-Phe-Ala-Lys-CMK; Protease Inhibitor Ac-Phe-Ala-Lys-CH2Cl; (2S)-6-Amino-2-[[(2S)-1-[[(2S)-3-(4-acetylphenyl)-1-chloro-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-N-(3-chloro-2-oxopropyl)hexanamide; APC-CMK; N-Acetyl-L-phenylalanyl-L-alanyl-N6-(3-chloro-2-oxopropyl)-L-lysine amide
EINECS	Contact for details

Quality Control

Our Acetylphenyl-Alanyl-Lysine Chloromethyl Ketone is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot undergoes comprehensive analytical characterization, including purity verification by HPLC, identity confirmation by mass spectrometry (MS) and infrared spectroscopy (IR), and impurity profiling. A detailed Certificate of Analysis (COA) documenting all test results is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.