Description

D-Val-Leu-Lys-Cmk is a potent, cell-permeable, and irreversible inhibitor of serine proteases, specifically targeting enzymes like trypsin and plasmin. This compound is a critical research tool for studying protease-mediated signaling pathways, cellular processes, and disease mechanisms. It is essential for pharmaceutical R&D, academic biochemistry research, and the development of novel therapeutic agents targeting proteolytic activity.

Application

• Biochemical Research: Used as a specific, irreversible inhibitor to study the function and inhibition kinetics of serine proteases like trypsin, plasmin, and related enzymes in vitro.
• Cell Biology Studies: Employed to investigate the role of specific proteases in cellular processes such as apoptosis, cell migration, and signal transduction due to its cell-permeable nature.
• Pharmaceutical Development: Serves as a key reference compound and pharmacological tool in the discovery and validation of new protease inhibitor drugs.
• Enzymology: Utilized in mechanistic studies to understand enzyme-substrate interactions and to develop assay protocols for high-throughput screening.
• Disease Model Research: Applied in models of inflammation, cancer, and cardiovascular diseases where dysregulated protease activity is a known pathological factor.

Basic Information

Product Name	D-Val-Leu-Lys-Cmk
CAS No.	75590-15-7
Molecular Formula	C22H41ClN6O4
Molecular Weight	488.06 g/mol
Synonyms	D-Valyl-L-leucyl-L-lysyl chloromethyl ketone; (2S)-6-Amino-2-[[(2S)-4-methyl-1-oxo-1-[[(2R)-3-methyl-1-oxobutan-2-yl]amino]pentan-2-yl]amino]-N-(chloromethyl)hexanamide; D-Val-Leu-Lys-CH2Cl; Boc-Val-Leu-Lys-CMK; Val-Leu-Lys Chloromethyl Ketone (D-Val isomer); Protease Inhibitor (Trypsin/Plasmin specific); Chloromethyl Ketone Inhibitor of Serine Proteases
EINECS	Contact for details

Quality Control

Our D-Val-Leu-Lys-Cmk is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and chiral analysis to ensure stereochemical integrity. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our stringent specifications for research and pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.