Description

Dphe-Phe-Arg-Chloromethylketone is a potent and selective synthetic peptide-based chloromethyl ketone inhibitor, widely recognized for its role in biochemical and pharmacological research. This compound is a critical tool for studying serine protease mechanisms, offering high specificity and irreversible binding to target enzymes. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic manufacturers focused on enzyme kinetics, drug discovery, and the development of therapeutic agents for conditions involving protease dysregulation.

Application

• Biochemical Research: As a key reagent for studying the activity, inhibition, and structure-function relationships of specific serine proteases.
• Pharmaceutical Development: Used in high-throughput screening and lead optimization for novel protease inhibitor drugs.
• Proteomics & Diagnostics: Employed in assay development and diagnostic kit manufacturing to detect and quantify protease activity.
• Academic Research: A standard tool in university and institutional labs for investigating blood coagulation, inflammation, and apoptosis pathways.
• Enzyme Mechanism Studies: Essential for elucidating catalytic mechanisms and designing active-site directed probes.

Basic Information

Product Name	Dphe-Phe-Arg-Chloromethylketone
CAS No.	74392-49-7
Molecular Formula	C31H42ClN7O5
Molecular Weight	628.16 g/mol
Synonyms	D-Phenylalanyl-L-phenylalanyl-L-arginine chloromethyl ketone; D-Phe-Phe-Arg-CH2Cl; D-Phe-Phe-Arg-CMK; FFrCK; Protease Inhibitor 74392-49-7; (2S)-5-[[(2S)-1-[[(2S)-1-[[(2R)-2-Amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-guanidino-1-oxobutan-2-yl]amino]-1-chloro-4-methylpentan-2-one
EINECS	Contact for details

Quality Control

Our Dphe-Phe-Arg-Chloromethylketone is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, MS for identity confirmation, and NMR for structural verification. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided to ensure the material meets the stringent requirements for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay	≥ 98%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.