Description

Phenylboronic Acid-Sepharose CAS NO 74188-11-7 is a specialized affinity chromatography medium where phenylboronic acid ligands are covalently immobilized onto a cross-linked Sepharose matrix. This product is engineered for the selective capture and purification of biomolecules containing cis-diol groups, such as glycoproteins, RNA, and catecholamines. It is an essential tool for researchers and process development scientists in the biotechnology, pharmaceutical, and diagnostic industries requiring high-resolution separation and analysis.

Application

• Glycoprotein Purification: Selective isolation and enrichment of glycosylated proteins, antibodies (e.g., mAbs), and other glycoconjugates from complex biological mixtures.
• Nucleic Acid Separation: Capture of RNA and DNA fragments containing terminal cis-diol groups for molecular biology and genomics applications.
• Diagnostic Assay Development: Used as a solid-phase capture reagent in ELISA and other immunoassay formats for detecting glycated biomarkers (e.g., HbA1c).
• Metabolite & Small Molecule Binding: Isolation of catecholamines (e.g., dopamine, epinephrine), nucleotides, and other cis-diol-containing metabolites from biological fluids.
• Process Chromatography: Scalable purification step in downstream bioprocessing for the manufacturing of therapeutic glycoproteins and vaccines.
• Lectins and Sugar-Binding Protein Studies: A complementary tool to lectin-affinity chromatography for studying carbohydrate-protein interactions.
• Sample Preparation for MS Analysis: Pre-fractionation and clean-up of glycopeptide and glycan samples prior to mass spectrometric analysis.

Basic Information

Product Name	Phenylboronic Acid-Sepharose
CAS No.	74188-11-7
Molecular Formula	N/A (Functionalized Polymer)
Molecular Weight	N/A (Matrix-Dependent)
Synonyms	PBA-Sepharose; Phenylboronic Acid Agarose; Boronate Affinity Gel; m-Aminophenylboronic Acid–Agarose; 3-Aminophenylboronic Acid Sepharose CL-4B; Boronic Acid Affinity Medium; cis-Diol Specific Adsorbent; Glycoprotein Binding Resin
EINECS	Contact for details

Quality Control

Our Phenylboronic Acid-Sepharose is manufactured under a quality management system. Each lot is tested to ensure consistent performance characteristics, including ligand density, binding capacity, and flow properties. Certificates of Analysis (COA) detailing lot-specific data are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in the supplied storage solution (typically 20% ethanol) to prevent microbial growth and maintain matrix stability. Do not freeze. For long-term storage, ensure the container is sealed to prevent evaporation.

Specification

Item	Specification
Matrix	Cross-linked Sepharose 4B
Ligand	m-Aminophenylboronic Acid
Ligand Density	Contact for details
Binding Capacity (for HbA1c)	Contact for details
Particle Size	45-165 μm
Maximum Flow Rate	Contact for details
pH Stability (long-term)	2-10
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.