Description

Tunicamycin B2 CAS NO 73942-09-3 is a specific congener of the tunicamycin family of nucleoside antibiotics, known for its potent inhibitory activity against N-linked glycosylation. This compound is a critical research tool for studying protein processing, secretion, and the unfolded protein response in eukaryotic cells. It is primarily utilized by researchers in pharmaceutical development, molecular biology, and biochemistry for investigating metabolic pathways and developing novel therapeutic strategies.

Application

• Biochemical Research: As a selective inhibitor of N-linked glycosylation to study protein folding, trafficking, and endoplasmic reticulum (ER) stress.
• Cancer Research: Investigation of anti-tumor activity and induction of apoptosis in various cancer cell lines by disrupting glycoprotein synthesis.
• Virology Studies: Research into viral replication mechanisms, as many viruses rely on host glycosylation machinery for proper envelope protein formation.
• Antibiotic Mechanism Studies: Serves as a reference standard for understanding the biological activity and biosynthesis of nucleoside antibiotics.
• Cell Biology: Tool for probing the role of glycosylation in cell adhesion, signaling, and differentiation processes.
• Pharmaceutical Screening: Used in high-throughput assays to identify compounds that modulate ER stress pathways or glycosylation inhibitors.

Basic Information

Product Name	Tunicamycin B2
CAS No.	73942-09-3
Molecular Formula	C39H64N4O17
Molecular Weight	860.94 g/mol
Synonyms	Tunicamycin B2; Antibiotic Tunicamycin B2; Tunicamycin II; NSC 177382; 10-[(2S,3S,4R,5R)-5-[(1S)-1,2-Dihydroxyethyl]-3,4-dihydroxyoxolan-2-yl]-5,6-dihydro-5-methyl-6-oxo-4H-pyrazolo[3,4-d]pyrimidine-4-carboxamide; Streptomyces lysosuperificus antibiotic B2; Glycosylation Inhibitor B2
EINECS	Contact for details

Quality Control

Our Tunicamycin B2 is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) documenting identity, purity, and potency is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Biological Activity	Inhibits protein glycosylation in cell-based assays
Solubility	Soluble in DMSO, methanol; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.