Description

Cortisol-9,11,12,12-D4 is a stable isotope-labeled analog of cortisol, a critical endogenous steroid hormone. This deuterated compound is essential as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and precision in analytical measurements. It is primarily required by pharmaceutical R&D laboratories, clinical research facilities, and contract research organizations (CROs) for method development, validation, and bioanalytical studies.

Application

• LC-MS/MS Internal Standard: Used as a critical reference standard for the precise quantification of endogenous cortisol levels in biological matrices such as plasma, serum, and urine.
• Clinical Diagnostics & Research: Enables accurate measurement of cortisol for studies related to stress response, adrenal function (e.g., Cushing's syndrome, Addison's disease), and circadian rhythm disorders.
• Pharmaceutical Development: Supports pharmacokinetic (PK) and pharmacodynamic (PD) studies for drug candidates targeting the hypothalamic-pituitary-adrenal (HPA) axis.
• Endocrinology & Metabolism Studies: Facilitates reliable analysis in research exploring steroidogenesis, metabolic pathways, and endocrine-disrupting chemicals.
• Doping Control & Forensic Analysis: Serves as a key component in confirmatory testing for synthetic glucocorticoids in sports anti-doping programs and forensic toxicology.
• Method Development & Validation: Provides a stable, non-interfering isotope pattern essential for developing robust, sensitive, and specific analytical methods in compliance with regulatory guidelines (e.g., FDA, EMA).

Basic Information

Product Name	Cortisol-9,11,12,12-D4
CAS No.	73565-87-4
Molecular Formula	C₂₁H₂₆D₄O₅
Molecular Weight	366.48 g/mol
Synonyms	11β,17α,21-Trihydroxypregn-4-ene-3,20-dione-9,11,12,12-d4; Hydrocortisone-d4; [9,11,12,12-²H₄]Cortisol; 4-Pregnene-11β,17α,21-triol-3,20-dione-9,11,12,12-d4; Deuterated Cortisol; Cortisol (9,11,12,12-d4); Stable Isotope-Labeled Cortisol
EINECS	Contact for details

Quality Control

Our Cortisol-9,11,12,12-D4 is manufactured under strict quality control protocols to ensure the highest standards of isotopic purity and chemical integrity. Each batch is characterized by advanced analytical techniques including NMR, HPLC, and high-resolution mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing isotopic enrichment (typically ≥98 atom % D), chemical purity (≥95% by HPLC), and confirming the absence of significant unlabeled impurities. Our quality system supports compliance with cGMP and ISO 9001 standards for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (Atom % D)	≥ 98%
Chemical Purity (by NMR)	Conforms
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.