Description

Calcimycin A23187 Hemimagnesium Salt is a high-purity, semi-synthetic derivative of the potent divalent cation ionophore A23187 (Calcimycin). This compound is specifically valued for its enhanced stability and defined stoichiometry, making it a critical tool for precise calcium and magnesium flux studies in biological research. It is primarily utilized by researchers in biochemistry, pharmacology, and cell biology for investigating intracellular signaling pathways, mitochondrial function, and ion transport mechanisms. The hemimagnesium salt form offers researchers a reliable and consistent reagent for modulating cation concentrations in experimental systems.

Application

• Biochemical Research: As a selective ionophore to manipulate intracellular Ca²⁺ and Mg²⁺ levels in cell culture and isolated organelle studies.
• Pharmacology & Drug Discovery: Used in assays to study the role of calcium signaling in cellular processes like apoptosis, secretion, and muscle contraction for target validation.
• Mitochondrial Function Analysis: Employed to induce mitochondrial permeability transition pore (MPTP) opening, a key event in studying cell death and bioenergetics.
• Cell Physiology Studies: A tool for probing the physiological effects of altered divalent cation homeostasis on enzyme activity and signal transduction.
• Reference Standard: Serves as a certified reference material (CRM) for analytical method development and quality control in the production of research biochemicals.

Basic Information

Product Name	Calcimycin A23187 Hemimagnesium Salt
CAS No.	72124-77-7
Molecular Formula	C₂₉H₃₇N₃O₆ • ½Mg
Molecular Weight	~ 532.0 g/mol (for hemimagnesium salt)
Synonyms	A23187 Hemimagnesium Salt; Calcimycin Hemimagnesium Salt; Antibiotic A23187 Hemimagnesium Salt; Calcium Ionophore A23187 Hemimagnesium Salt; 5-(Methylamino)-2-[[(2S,3R,5S,6R,8S,9R)-3,5,6,9-tetramethyl-2-[(1-oxohexyl)oxy]-1,7-dioxaspiro[5.5]undec-8-yl]methyl]-4-benzoxazolecarboxylic acid hemimagnesium salt
EINECS	Contact for details

Quality Control

Our Calcimycin A23187 Hemimagnesium Salt is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by spectroscopic methods (IR, NMR), and specific tests for residual solvents and heavy metals. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Solvent Residues (GC)	Meets ICH guidelines
Biological Activity	Tested in a standard ionophore assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.