Description

Z-Phe-Ala-Diazomethylketone is a specialized, high-purity peptide-based diazomethyl ketone, a potent and irreversible inhibitor of cysteine proteases. This compound is critical for researchers and manufacturers requiring precise enzyme inhibition in biochemical pathways. It is primarily utilized in pharmaceutical R&D, proteomics research, and as a key intermediate in the synthesis of more complex therapeutic agents targeting specific enzymatic activity.

Application

• Cysteine Protease Inhibition Research: Serves as a potent, irreversible active-site directed inhibitor for enzymes like cathepsins B, L, and S in mechanistic and structural studies.
• Pharmaceutical Development: Used as a key pharmacophore or intermediate in the design and synthesis of novel drug candidates targeting protease-related diseases (e.g., osteoporosis, cancer, parasitic infections).
• Biochemical Assay Development: Employed as a standard or tool compound to validate assay systems and screen for new protease inhibitors in high-throughput formats.
• Proteomics & Cell Biology: Applied to study the role of specific cysteine proteases in cellular processes like protein turnover, apoptosis, and immune response.
• Chemical Biology Probe Synthesis: Acts as a versatile building block for creating activity-based probes (ABPs) to profile protease activity in complex biological samples.

Basic Information

Product Name	Z-Phe-Ala-Diazomethylketone
CAS No.	71732-53-1
Molecular Formula	C22H24N4O5
Molecular Weight	424.45 g/mol
Synonyms	Z-Phe-Ala-CHN2; Benzyloxycarbonyl-Phenylalanyl-Alanyl-Diazomethane; Z-Phe-Ala-DMK; CBZ-Phe-Ala-Diazomethyl Ketone; N-CBZ-L-Phenylalanyl-L-Alanyl Diazomethyl Ketone; Protease Inhibitor Z-Phe-Ala-CHN2; Cathepsin Inhibitor Z-Phe-Ala-Diazomethane; (2S)-2-[[(2S)-3-Phenyl-2-[(phenylmethoxy)carbonylamino]propanoyl]amino]-N-(diazomethyl)-N-oxopropylamide
EINECS	Contact for details

Quality Control

Our Z-Phe-Ala-Diazomethylketone is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.