Description

Gly-Asp-Asp-Asp-Asp-Lys β-Naphthylamide is a specialized synthetic peptide substrate designed for high-precision enzymatic assays. This compound is critical for researchers and manufacturers requiring accurate detection and quantification of specific protease activity, particularly in complex biological matrices. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, biochemical research, and quality control of therapeutic enzymes.

Application

• Protease Activity Assays: As a fluorogenic substrate for the specific detection and kinetic analysis of proteolytic enzymes, including trypsin-like and other serine proteases.
• Biomedical Research: Used in studying enzyme kinetics, inhibitor screening, and characterizing protease function in cellular pathways and disease models.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and validate novel protease inhibitors as potential drug candidates.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical laboratories to measure specific enzyme levels.
• Biochemical Reagent: Serves as a critical reagent in academic and industrial laboratories for standardizing enzymatic methods and assays.
• Quality Control: Used to assess the purity and specific activity of commercial enzyme preparations in biomanufacturing.

Basic Information

Product Name	Gly-Asp-Asp-Asp-Asp-Lys β-Naphthylamide
CAS No.	70023-02-8
Molecular Formula	C43H58N10O16
Molecular Weight	971.0 g/mol
Synonyms	GDDDDK-βNA; H-Gly-Asp-Asp-Asp-Asp-Lys-β-naphthylamide; Glycyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-lysyl-2-naphthylamide; Fluorogenic Peptide Substrate GDDDDK-βNA; Protease Substrate 70023-02-8; L-Lysine, N2-[N-[N-[N-[N-(1-oxo-2-glycyl-L-α-aspartyl)-L-α-aspartyl]-L-α-aspartyl]-L-α-aspartyl]-, 1-(2-naphthyl) ester
EINECS	Contact for details

Quality Control

Every batch of Gly-Asp-Asp-Asp-Asp-Lys β-Naphthylamide is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and performance consistency. Our quality system is designed to meet the stringent requirements of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), identity confirmation, and other critical parameters is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference standard in assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.