Description

Nutlin 3A is a potent, cell-permeable small molecule antagonist of the MDM2-p53 protein-protein interaction, functioning as a cis-imidazoline analog. This compound is a critical research tool for selectively stabilizing and activating the p53 tumor suppressor pathway in cancer biology and drug discovery studies. It is essential for scientists and pharmaceutical developers in oncology research, high-throughput screening, and the development of novel cancer therapeutics targeting the p53-MDM2 axis.

Application

• Oncology Research & Drug Discovery: A key pharmacological tool for studying p53-dependent apoptosis, cell cycle arrest, and senescence in various cancer cell lines.
• Biochemical Assay Development: Used as a reference standard and positive control in fluorescence polarization (FP), surface plasmon resonance (SPR), and other binding assays targeting MDM2.
• Mechanistic Studies: Enables investigation of the therapeutic potential of disrupting the p53-MDM2/MDMX interaction for cancer treatment.
• High-Throughput Screening (HTS): Serves as a benchmark compound in screening campaigns to identify new MDM2 inhibitors.
• Preclinical Studies: Utilized in in vitro and in vivo models to evaluate antitumor efficacy and pharmacodynamics.
• Chemical Biology: Employed to probe protein-protein interaction networks and validate targets in cellular pathways.

Basic Information

Product Name	Nutlin 3A
CAS No.	675576-98-4
Molecular Formula	C30H30Cl2N4O4
Molecular Weight	581.49 g/mol
Synonyms	Nutlin-3a; (-)-Nutlin 3; Nutlin-3A; (R)-Nutlin-3; 4-[(4S,5R)-4,5-Bis(4-chlorophenyl)-4,5-dihydro-2-[4-methoxy-2-(1-methylethoxy)phenyl]-1H-imidazol-1-yl]carbonyl-2-piperazinone; 4-[(4S,5R)-4,5-Bis(4-chlorophenyl)-2-[2-isopropoxy-4-methoxyphenyl]-4,5-dihydro-1H-imidazole-1-carbonyl]piperazin-2-one; MDM2 antagonist; cis-Imidazoline analog
EINECS	Contact for details

Quality Control

Our Nutlin 3A is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency for research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, NMR for structural confirmation, and chiral analysis to verify enantiomeric excess. A detailed Certificate of Analysis (COA) containing all specification results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under inert atmosphere. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Chiral Purity (HPLC)	≥99.0% ee
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.