Description

Nutlin-3B is a potent and selective small-molecule inhibitor of the MDM2-p53 protein-protein interaction, serving as a negative control for the active enantiomer Nutlin-3. This compound is a critical research tool for studying the p53 tumor suppressor pathway and its role in cellular processes like apoptosis and cell cycle arrest. It is essential for pharmaceutical R&D, academic research, and biotechnology companies focused on oncology, cancer biology, and novel therapeutic discovery.

Application

• Biomedical Research: Used as a key negative control in studies investigating the specific effects of MDM2 inhibition on p53 activation and downstream signaling.
• Oncology & Cancer Biology: Fundamental for in vitro and in vivo experiments to understand tumor suppression mechanisms and validate the specificity of active p53 pathway modulators.
• Drug Discovery & Development: Employed in high-throughput screening assays and mechanistic studies to identify and characterize new anticancer agents targeting the MDM2-p53 axis.
• Academic & Institutional Laboratories: A standard reagent for basic research into cell death, DNA damage response, and cell cycle regulation.
• Pharmaceutical R&D: Supports preclinical development by helping to delineate on-target versus off-target effects of candidate therapeutics.

Basic Information

Product Name	Nutlin-3B
CAS No.	675576-97-3
Molecular Formula	C30H30Cl2N4O4
Molecular Weight	581.49 g/mol
Synonyms	Nutlin-3B; (-)-Nutlin-3; Nutlin 3B; (R)-Nutlin-3; 4-[(4R,5R)-4,5-Bis(4-chlorophenyl)-4,5-dihydro-2-[4-methoxy-2-(1-methylethoxy)phenyl]-1H-imidazol-1-yl]carbonyl]morpholine; MDM2 antagonist negative control; p53-MDM2 interaction inhibitor negative control
EINECS	Contact for details

Quality Control

Our Nutlin-3B is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and residual solvent analysis. A Certificate of Analysis (COA) detailing batch-specific results is provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular structure
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Heavy Metals	≤20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.