Description

(+/-)-Blebbistatin CAS NO 674289-55-5 is a potent, cell-permeable, and selective inhibitor of non-muscle myosin II ATPase activity. This compound matters significantly as a critical research tool for studying cytoskeletal dynamics, cell division, and cell migration processes. It is essential for researchers and scientists in the fields of cell biology, pharmacology, and biomedical research, particularly for investigating myosin II's role in cytokinesis, neuronal growth cone dynamics, and cancer cell metastasis.

Application

• Cytoskeleton Research: A primary tool for inhibiting non-muscle myosin II to study actin-myosin contractility in cell division (cytokinesis) and cell motility.
• Neuroscience Studies: Used to investigate the role of myosin II in neuronal growth cone guidance, axon retraction, and synaptic plasticity.
• Cancer Research: Employed to probe the mechanisms of tumor cell invasion and metastasis, which are driven by actomyosin contractility.
• Cardiovascular Research: Applied in studies of smooth muscle contraction and endothelial cell barrier function.
• Developmental Biology: Utilized to understand morphogenetic movements and tissue patterning during embryogenesis.
• High-Content Screening (HCS): Serves as a reference compound in phenotypic screening assays targeting the cytoskeleton.
• Biochemical Assays: Acts as a specific inhibitor in in vitro ATPase activity assays for characterizing myosin II enzymes.

Basic Information

Product Name	(+/-)-Blebbistatin
CAS No.	674289-55-5
Molecular Formula	C18H16N2O3
Molecular Weight	308.33 g/mol
Synonyms	Blebbistatin (racemic); (±)-Blebbistatin; 3-(2,4-Dichlorophenyl)-4-(1H-pyrrolo[2,3-b]pyridin-4-yl)-1H-pyrrole-2,5-dione; Myosin II Inhibitor V; NSC 761125; Bleb; B5924 (σ-Aldrich code)
EINECS	Contact for details

Quality Control

Our (+/-)-Blebbistatin is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity determination and structural confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and other critical parameters.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. Allow the product to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to light brown solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.