Description

Ldn-57444 is a potent and selective small molecule inhibitor of the deubiquitinating enzyme USP1 (Ubiquitin Specific Peptidase 1). This compound is a critical research tool for investigating the role of the USP1/UAF1 complex in DNA damage response and repair pathways. It is primarily utilized by researchers and scientists in the fields of oncology, molecular biology, and biochemistry for target validation, mechanism-of-action studies, and high-throughput screening. The product is supplied as a high-purity biochemical reagent to ensure reliable and reproducible experimental results.

Application

• Biochemical Research: Used as a selective inhibitor to study the function and mechanism of the USP1/UAF1 deubiquitinating complex in vitro.
• Oncology Research: Investigated for its potential to sensitize cancer cells, particularly in Fanconi anemia pathway-deficient cancers, to DNA-damaging agents like cisplatin.
• Cell Biology Studies: Applied in cell-based assays to probe DNA repair mechanisms, cell cycle regulation, and genomic stability.
• Target Validation: Serves as a pharmacological tool to validate USP1 as a therapeutic target in various disease models.
• High-Throughput Screening (HTS): Used as a reference compound in screening campaigns to discover novel modulators of the ubiquitin-proteasome system.
• Academic & Pharmaceutical R&D: Essential for basic research and early-stage drug discovery programs focused on DNA repair and cancer therapeutics.

Basic Information

Product Name	Ldn-57444
CAS No.	668467-91-2
Molecular Formula	C₁₉H₁₈N₄O₃
Molecular Weight	350.37 g/mol
Synonyms	LDN-57444; LDN57444; USP1 Inhibitor; 4-[(4-Methylphenyl)sulfonyl]-1,2,3,4-tetrahydro-γ-carboline-3-carboxylic acid; 1,2,3,4-Tetrahydro-4-[(4-methylphenyl)sulfonyl]-9H-pyrido[3,4-b]indole-3-carboxylic acid; γ-Carboline-3-carboxylic acid, 1,2,3,4-tetrahydro-4-[(4-methylphenyl)sulfonyl]-; USP1/UAF1 Inhibitor
EINECS	Contact for details

Quality Control

Our Ldn-57444 is manufactured under strict quality control protocols to ensure high chemical purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is accompanied by comprehensive analytical data, including HPLC purity, NMR, and mass spectrometry confirmation. Certificates of Analysis (COA) are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.