Description

n-Hydroxyphenacetin Glucuronide is a key metabolite of phenacetin, a compound of significant interest in pharmacological and toxicological research. This glucuronide conjugate is essential for studying drug metabolism pathways, bioactivation mechanisms, and the pharmacokinetic profiles of analgesic compounds. It serves as a critical reference standard and analytical tool for researchers and quality control laboratories in the pharmaceutical, contract research organization (CRO), and academic sectors, particularly those focused on ADME (Absorption, Distribution, Metabolism, and Excretion) studies and metabolite identification.

Application

• Pharmaceutical Reference Standard: Used as a high-purity certified reference material (CRM) for the quantification and qualification of phenacetin metabolites in biological matrices.
• Drug Metabolism & Pharmacokinetics (DMPK): A vital tool in in vitro and in vivo studies to elucidate metabolic pathways, enzyme kinetics, and the formation of reactive intermediates.
• Toxicological Research: Employed in investigations into the nephrotoxic and carcinogenic potential associated with phenacetin usage and its metabolic bioactivation.
• Analytical Method Development: Serves as a critical standard for developing and validating sensitive LC-MS/MS, HPLC, and other chromatographic assays in bioanalytical laboratories.
• Forensic and Clinical Toxicology: Used in assays to monitor drug abuse or historical exposure to phenacetin-containing formulations.
• Academic Research: Supports fundamental research in medicinal chemistry, biochemistry, and pharmacology related to phase II conjugation reactions and drug detoxification processes.

Basic Information

Product Name	n-Hydroxyphenacetin Glucuronide
CAS No.	69783-19-3
Molecular Formula	C18H21NO9
Molecular Weight	395.36 g/mol
Synonyms	N-(4-Ethoxyphenyl)acetamide Glucuronide (N-Hydroxy); 4'-Hydroxyphenacetin O-Glucuronide; Phenacetin Metabolite Glucuronide; N-Hydroxy Phenacetin Glucuronide; Acetophenetidin Metabolite; 1-N-Hydroxyphenacetin β-D-Glucopyranosiduronic Acid; N-Acetyl-p-aminophenol O-Glucuronide (N-Hydroxy derivative); p-Ethoxyacetanilide N-Hydroxy Glucuronide
EINECS	Contact for details

Quality Control

Our n-Hydroxyphenacetin Glucuronide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and residual solvent analysis, to ensure it meets the exacting standards required for research and reference applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and analytical methods is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere or with desiccant to maintain stability and purity. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.