Description

Cm-Sepharose(R) Cl-6B, For Chromato- Graphy* is a high-performance cation exchange chromatography medium based on cross-linked 6% agarose beads, functionalized with carboxymethyl groups. This product is essential for the purification and separation of a wide range of biomolecules, including proteins, peptides, and nucleic acids, based on their net positive charge under specific buffer conditions. It is a critical tool for researchers and process development scientists in biopharmaceutical manufacturing, academic research, and diagnostic kit production who require reliable, high-capacity media for downstream processing.

Application

• Purification of therapeutic proteins and antibodies in biopharmaceutical downstream processing.
• Fractionation of complex protein mixtures in academic and industrial research laboratories.
• Separation of enzymes and other cationic biomolecules for biochemical analysis and assay development.
• Capture and initial purification step in multi-column chromatography processes.
• Polishing step to remove host cell proteins and other charged impurities.
• Process development and scale-up studies for manufacturing optimization.

Basic Information

Product Name	Cm-Sepharose(R) Cl-6B, For Chromato- Graphy*
CAS No.	68894-07-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cm-Sepharose CL-6B; Carboxymethyl Sepharose CL-6B; CM Sepharose CL-6B; Sepharose CL-6B CM; Cation Exchanger Sepharose; Agarose CM Ion Exchanger; Cross-linked CM-Sepharose 6B; Weak Cation Exchange Chromatography Medium
EINECS	Contact for details

Quality Control

Our Cm-Sepharose CL-6B is manufactured under a rigorous quality management system. Each lot is tested to ensure consistent performance characteristics, including ligand density, bead size distribution, flow properties, and binding capacity for standard proteins. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (36-46°F) in the supplied storage solution (typically 20% ethanol). Do not freeze. Keep the container tightly sealed to prevent evaporation and microbial contamination.

Specification

Item	Specification
Matrix	Cross-linked 6% Agarose
Functional Group	Carboxymethyl (CM)
Ion Capacity	Contact for details
Bead Size Range	45-165 μm
Maximum Operating Pressure	0.3 MPa, 3 bar
pH Stability	2-13 (long term), 3-12 (working)
Storage Solution	20% Ethanol
Binding Capacity (for Lysozyme)	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.