Description

4-Cholesten-3-One-4-13C is a stable isotope-labeled analog of cholestenone, specifically enriched with carbon-13 at the 4-position. This compound serves as a critical internal standard and tracer in metabolic and biochemical research, enabling precise quantification and pathway analysis. It is essential for researchers and manufacturers in the pharmaceutical, academic, and analytical chemistry sectors who require high-purity, isotopically labeled sterols for advanced studies in lipid metabolism, steroid biosynthesis, and mass spectrometry.

Application

• Internal Standard for Mass Spectrometry (MS): Used as a quantitative reference in LC-MS and GC-MS analysis of sterols and related metabolites to ensure accuracy and reproducibility.
• Metabolic Pathway Tracing: Employed in isotope-labeling studies to elucidate biosynthetic pathways and metabolic fluxes of cholesterol and steroid hormones.
• Pharmaceutical Research & Development: Facilitates drug metabolism and pharmacokinetic (DMPK) studies for compounds targeting lipid-related disorders.
• Biochemical Research: Acts as a precursor or intermediate in the synthesis of other labeled steroid compounds for mechanistic studies.
• Quality Control & Reference Material: Serves as a certified reference material (CRM) for calibrating analytical instruments and validating methods in quality control laboratories.

Basic Information

Product Name	4-Cholesten-3-One-4-13C
CAS No.	68165-57-1
Molecular Formula	C2713CH44O
Molecular Weight	~385.65 g/mol
Synonyms	Cholest-4-en-3-one-4-13C; 4-Cholesten-3-one-4-(13C); 5-Cholesten-3-one-4-13C; δ4-Cholesten-3-one-4-13C; 4-13C-Cholestenone; [4-13C]-Cholest-4-en-3-one; Isotopically Labeled Cholestenone
EINECS	Contact for details

Quality Control

Our 4-Cholesten-3-One-4-13C is manufactured under strict quality systems to ensure high isotopic and chemical purity, meeting the exacting requirements of research and analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment, chemical purity (by HPLC), and identity confirmation. We adhere to relevant industry standards for reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry place. The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C
Chemical Purity	≥ 95%
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.