Description

4-Bromo-2,3-Dioxobutyl-Coenzyme A is a specialized biochemical reagent and a key synthetic intermediate in the study of enzyme mechanisms and metabolic pathways. This compound matters for its role as a mechanism-based inhibitor and a substrate analog, enabling critical research in enzymology and biochemistry. It is primarily needed by research institutions, pharmaceutical R&D departments, and biotechnology companies focused on metabolic engineering, drug discovery, and fundamental biochemical studies.

Application

• Enzymology Research: Used as a mechanism-based inhibitor to study the active sites and catalytic mechanisms of specific CoA-dependent enzymes.
• Metabolic Pathway Analysis: Serves as a substrate analog to probe and elucidate complex metabolic networks and flux in cellular systems.
• Pharmaceutical Development: Acts as a crucial intermediate in the synthesis of novel drug candidates targeting metabolic disorders.
• Biochemical Assay Development: Employed in the development of high-throughput screening assays for enzyme activity and inhibitor discovery.
• Academic Research: A vital tool for universities and research institutes conducting fundamental studies in biochemistry and molecular biology.
• Biocatalyst Engineering: Used in studies aimed at engineering enzymes for industrial biocatalysis and synthetic biology applications.

Basic Information

Product Name	4-Bromo-2,3-Dioxobutyl-Coenzyme A
CAS No.	66686-64-4
Molecular Formula	C25H40BrN7O17P3S
Molecular Weight	908.51 g/mol
Synonyms	4-Bromo-2,3-dioxobutyl-CoA; 4-Bromoacetoacetyl-CoA; Bromoacetoacetyl coenzyme A; 4-Bromo-2,3-dioxobutyl-coenzyme A; 4-Bromoacetoacetyl coenzyme A; 3-Bromo-2,4-dioxobutyl-CoA (tautomer); CoA-bromoacetoacetate; Bromoacetoacetyl-CoA thioester
EINECS	Contact for details

Quality Control

Our 4-Bromo-2,3-Dioxobutyl-Coenzyme A is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity, essential for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the vial to reach room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Biological Activity	Confirmed in enzymatic assay (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.