Description

Tunicamycin B Complex is a potent mixture of nucleoside antibiotics that acts as a specific inhibitor of N-linked glycosylation. This compound is a critical research tool for studying protein processing, secretion, and the unfolded protein response in eukaryotic cells. It is essential for scientists in pharmaceutical R&D, cell biology, and biochemistry who are investigating metabolic pathways, cancer biology, and the mechanisms of endoplasmic reticulum stress.

Application

• Biochemical Research: A key reagent for inhibiting protein glycosylation in cell culture and in vitro studies to elucidate glycoprotein function.
• Cancer Research: Used to induce endoplasmic reticulum stress and study apoptosis in various cancer cell lines.
• Antiviral Studies: Employed to investigate the role of glycosylation in viral envelope protein maturation and infectivity.
• Cell Signaling Pathways: A tool for dissecting the unfolded protein response (UPR) and its role in cellular homeostasis and disease.
• Antibiotic Mechanism Studies: Serves as a reference standard in microbiological research on nucleoside antibiotics.
• Pharmaceutical Development: Used in target validation and screening assays for diseases related to protein misfolding.

Basic Information

Product Name	Tunicamycin B Complex
CAS No.	66081-36-5
Molecular Formula	C39H64N4O16 (for Tunicamycin B1)
Molecular Weight	844.95 g/mol (for Tunicamycin B1)
Synonyms	Tunicamycin B; Tunicamycin B Mixture; Antibiotic Tunicamycin B Complex; Tunicamycin from *Streptomyces lysosuperificus*; NSC 177382; Uridine, 5'-O-[N-acetyl-α-D-glucosaminyl-1-dihydrogen pyrophosphoryl]-2'-(12-methyltridecanoylamino)-2'-deoxy-; Tunicamycin B1, B2, B3, B4 Complex
EINECS	Contact for details

Quality Control

Our Tunicamycin B Complex is produced and handled under strict quality protocols to ensure batch-to-batch consistency and biological activity. Each lot is characterized by advanced analytical techniques, including HPLC and mass spectrometry, to confirm the composition of the B complex. A comprehensive Certificate of Analysis (COA) detailing purity, potency, and identification is provided with every shipment to support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard profile
Purity (HPLC)	≥ 95% (Total Tunicamycin B Complex)
Potency (Microbiological)	≥ 980 µg/mg
Water Content (KF)	≤ 2.0 %
Solubility	Soluble in DMSO, Methanol; Slightly soluble in Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.