Description

5-Fluorouracil Glucuronide is a key metabolite and prodrug derivative of the widely used chemotherapeutic agent 5-fluorouracil (5-FU). This compound is of significant interest in pharmaceutical research and development due to its role in the metabolic pathway and potential for targeted drug delivery, which can help mitigate systemic toxicity. It is primarily utilized by research institutions, pharmaceutical companies, and contract development and manufacturing organizations (CDMOs) engaged in oncology drug discovery, pharmacokinetic studies, and the synthesis of novel therapeutic conjugates.

Application

• Pharmaceutical Reference Standard: Serves as a critical analytical standard for quantifying 5-FU and its metabolites in bioanalytical studies (e.g., plasma, urine) to support pharmacokinetic and bioequivalence assessments.
• Oncology Research: Used as a biochemical tool to investigate the metabolism, activation, and resistance mechanisms of fluoropyrimidine-based chemotherapy in cancer cell lines and animal models.
• Prodrug Development: Acts as a synthetic intermediate or model compound in the design and synthesis of novel glucuronide prodrugs aimed at achieving tumor-selective activation.
• Enzyme Substrate Studies: Employed as a substrate for β-glucuronidase enzymes in biochemical assays to study enzyme kinetics and inhibition, relevant for antibody-directed enzyme prodrug therapy (ADEPT).
• Metabolite Identification & Synthesis: Essential for the synthesis and characterization of metabolite markers used in drug metabolism and pharmacodynamics (DMPK) studies during preclinical and clinical development.
• Quality Control: Used by API manufacturers as a system suitability standard in HPLC/UPLC methods to ensure the purity and identity of 5-FU active pharmaceutical ingredients (APIs) and related substances.

Basic Information

Product Name	5-Fluorouracil Glucuronide
CAS No.	66048-45-1
Molecular Formula	C11H13FN2O9
Molecular Weight	336.23 g/mol
Synonyms	5-FU Glucuronide; 1-((3,4,5-Trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-5-fluoro-1,2,3,4-tetrahydropyrimidine-2,4-dione; 5-Fluoro-1-(β-D-glucopyranuronosyl)uracil; β-1-Glucuronosyl-5-fluorouracil; 5-Fluorouridine Glucuronide (ambiguous); 5-Fluorouracil-1-glucuronide; GLU-5FU
EINECS	Contact for details

Quality Control

Our 5-Fluorouracil Glucuronide is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC purity assay, identification by spectroscopic methods (IR, NMR, MS), and control of specified impurities. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting the results against our stringent in-house specifications. We support research and development with materials suitable for use as reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Complies with ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.