Description

D-Phe-Pro-Arg-Chloromethylketone CAS NO 65149-23-7 is a potent and selective synthetic tripeptide chloromethyl ketone inhibitor of serine proteases. This compound is a critical research tool for studying and controlling coagulation pathways, particularly thrombin activity. It is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors who require high-purity biochemical inhibitors for assay development, drug discovery, and mechanistic studies.

Application

• Biochemical Research: As a specific active-site inhibitor for mechanistic studies of thrombin and related serine proteases.
• Pharmaceutical Development: Used in the discovery and validation of novel anticoagulant and anti-thrombotic drug candidates.
• Diagnostic Reagent Manufacturing: A key component in the formulation of control reagents and calibrators for coagulation testing kits.
• Enzymology Studies: For the kinetic characterization and inhibition profiling of trypsin-like enzymes.
• Cell Biology & Proteomics: To inhibit specific protease activities in cell lysates and complex biological samples.
• Reference Standard: Serves as a high-purity analytical standard for quality control in related product manufacturing.

Basic Information

Product Name	D-Phe-Pro-Arg-Chloromethylketone
CAS No.	65149-23-7
Molecular Formula	C23H33ClN6O4
Molecular Weight	505.00 g/mol
Synonyms	PPACK; D-Phenylalanyl-L-prolyl-L-arginine Chloromethyl Ketone; D-Phe-Pro-Arg-CH2Cl; Thrombin Inhibitor I; (2R)-2-[[(2S)-1-[[(2S)-5-(diaminomethylideneamino)-1-[(2-chloroacetyl)amino]pentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-phenylpropanoic acid; PPACK-Thrombin Inhibitor; Argatroban-related inhibitor
EINECS	Contact for details

Quality Control

Our D-Phe-Pro-Arg-Chloromethylketone is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture content. We provide full traceability and Certificates of Analysis (COA) are available upon request to ensure compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.