Description

Glycocholic Acid-13C1 (Glycyl-1-13C) is a stable isotope-labeled analog of the primary bile acid, glycocholic acid, where the glycine moiety is specifically enriched with carbon-13 at the 1-position. This compound is essential as an internal standard for the precise quantification and metabolic profiling of bile acids in complex biological matrices using advanced analytical techniques like LC-MS/MS. It is a critical reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors, enabling accurate studies of liver function, digestive disorders, and drug metabolism. The product is supplied with the CAS Registry Number 64431-95-4.

Application

• Mass Spectrometry Internal Standard: Serves as a critical quantitative reference for the analysis of endogenous glycocholic acid and related bile acids in bioanalytical assays.
• Metabolic Pathway Tracing: Used in in vitro and in vivo studies to investigate bile acid synthesis, enterohepatic circulation, and metabolic flux.
• Clinical Diagnostics: Enables the development and validation of highly accurate LC-MS/MS methods for diagnosing hepatobiliary diseases and disorders of lipid metabolism.
• Pharmaceutical R&D: Facilitates drug metabolism and pharmacokinetic (DMPK) studies by allowing precise measurement of bile acid displacement or modulation by new chemical entities.
• Quality Control & Method Validation: Essential for ensuring accuracy, precision, and compliance in regulated laboratory environments following GLP/GMP guidelines.
• Nutritional & Gut Microbiome Research: Aids in studying the interaction between diet, gut flora, and bile acid pool composition.

Basic Information

Product Name	Glycocholic Acid-13C1 (Glycyl-1-13C)
CAS No.	64431-95-4
Molecular Formula	C26H43NO6 (with 13C at specified position)
Molecular Weight	~466.6 g/mol (13C adjusted)
Synonyms	N-[(3α,5β,7α,12α)-3,7,12-Trihydroxy-24-oxocholan-24-yl]glycine-1-13C; Cholylglycine-1-13C; Glycocholate-13C1; 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine-1-13C; N-(3α,7α,12α-Trihydroxy-5β-cholan-24-oyl)glycine-1-13C; Taurocholic Acid-13C1 (related compound); Stable Isotope Labeled Bile Acid; 13C-Glycocholic Acid.
EINECS	Contact for details

Quality Control

Every batch of Glycocholic Acid-13C1 is manufactured and analyzed under strict quality systems to ensure identity, purity, and isotopic enrichment meet the exacting standards required for analytical applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request and provide detailed data for critical parameters including isotopic purity (atom % 13C), chemical purity (HPLC), and confirmation of structure (NMR/MS).

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant in the container. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (Atom % 13C)	≥ 98%
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.