Description

Necrostatin-1, Inactive Control CAS NO 64419-92-7 is a critical negative control compound used in biochemical and pharmacological research to validate the specific activity of Necrostatin-1. This compound is essential for distinguishing between on-target effects and non-specific cellular responses in studies of necroptosis, a programmed form of cell death. It is a fundamental tool for researchers in academic institutions, pharmaceutical R&D, and biotechnology companies focused on cell death pathways, inflammation, and neurodegenerative diseases.

Application

• Necroptosis Research Control: Serves as a vital negative control in experiments to confirm that observed inhibitory effects are specifically due to Necrostatin-1 and not off-target interactions.
• Biochemical Assay Validation: Used to establish baseline activity and specificity in high-throughput screening (HTS) campaigns targeting the RIPK1 kinase pathway.
• Pharmacology Studies: Essential for dose-response studies and determining the IC50 of active compounds by providing a reference for non-specific binding or cytotoxicity.
• Cell Culture Experiments: Applied in in vitro models of ischemia-reperfusion injury, neurodegenerative conditions, and inflammatory diseases to rule out assay artifacts.
• Mechanism of Action Elucidation: Helps researchers delineate the precise molecular mechanisms of necroptosis inhibitors in complex biological systems.
• Drug Discovery & Development: A standard reagent in the preclinical pipeline for developing novel therapeutics targeting regulated cell death.

Basic Information

Product Name	Necrostatin-1, Inactive Control
CAS No.	64419-92-7
Molecular Formula	C₁₃H₁₃N₃O₂S
Molecular Weight	275.33 g/mol
Synonyms	5-(1H-Indol-3-ylmethyl)-3-methyl-2-thioxo-4-imidazolidinone; 5-(Indol-3-ylmethyl)-3-methyl-2-thiohydantoin; Nec-1, Inactive Control; (5Z)-5-(1H-Indol-3-ylmethylene)-3-methyl-2-sulfanylidene-4-imidazolidinone (Inactive Form); Necroptosis Inhibitor Negative Control; RIPK1 Inhibitor Control Compound; Methyl-thiohydantoin indole derivative
EINECS	Contact for details

Quality Control

Our Necrostatin-1, Inactive Control is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity confirmation and structural verification by spectroscopic methods, to ensure identity, potency, and consistency. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (IR)	Spectrum matches reference material
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.