Description

4Alpha-Phorbol 12-Myristate 13-Acetate (CAS NO 63597-44-4) is a potent and specific activator of protein kinase C (PKC), widely recognized as a critical biochemical tool in life science research. Its primary value lies in its ability to reliably induce cellular responses, making it indispensable for studying signal transduction pathways, immune cell activation, and tumor promotion mechanisms. This high-purity compound is essential for researchers and developers in pharmaceutical R&D, immunology, and oncology, where precise and reproducible biological activity is paramount.

Application

• Biomedical Research: Used as a standard PKC activator to study intracellular signaling cascades, gene expression, and cell proliferation/differentiation.
• Immunology Studies: Employed to stimulate T-cells, neutrophils, and other immune cells, facilitating research into inflammation and immune response mechanisms.
• Cancer Research: A key tool in investigating tumor promotion, studying the role of PKC in carcinogenesis and testing potential chemopreventive agents.
• Drug Discovery & Screening: Utilized in high-throughput screening assays to identify and validate novel PKC inhibitors or modulators for therapeutic development.
• Cell Culture & Differentiation: Applied in vitro to induce specific differentiation pathways in various cell lines, including myeloid leukemia cells.
• Toxicology & Safety Assessment: Serves as a positive control in studies assessing skin irritation and phototoxicity.

Basic Information

Product Name	4Alpha-Phorbol 12-Myristate 13-Acetate
CAS No.	63597-44-4
Molecular Formula	C36H56O8
Molecular Weight	616.83 g/mol
Synonyms	PMA; Phorbol 12-Myristate 13-Acetate; TPA; Tetradecanoylphorbol Acetate; 12-O-Tetradecanoylphorbol-13-acetate; NSC 350836; 4α-Phorbol 12-myristate 13-acetate; Phorbol myristate acetate
EINECS	Contact for details

Quality Control

Our 4Alpha-Phorbol 12-Myristate 13-Acetate is manufactured under strict quality management systems to ensure the high purity and biological activity required for sensitive research applications. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing batch-specific results is provided to guarantee traceability and compliance with your research standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider under an inert atmosphere. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Solubility	Soluble in DMSO, ethanol, acetone
Biological Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.