Description

Leucine Chloromethyl Ketone is a specialized biochemical reagent and a potent, irreversible inhibitor of serine proteases. Its core value lies in its ability to selectively and covalently modify active sites, making it an essential tool for enzyme mechanism studies and proteomics research. This compound is primarily needed by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in drug discovery and biochemical pathway analysis.

Application

• Protease Inhibition Studies: Used as a specific active-site directed inhibitor for studying the function and mechanism of serine proteases like trypsin, chymotrypsin, and elastase.
• Biochemical Research: A critical reagent for labeling and identifying active serine residues in enzymes, aiding in structural and functional proteomics.
• Pharmaceutical Development: Employed in early-stage drug discovery to validate protease targets and develop inhibitor-based therapeutic strategies.
• Peptide Synthesis & Modification: Serves as an alkylating agent for the selective modification of peptides and proteins in synthetic chemistry applications.
• Cell Biology: Utilized to investigate the role of specific proteases in cellular processes such as apoptosis, signal transduction, and inflammation.

Basic Information

Product Name	Leucine Chloromethyl Ketone
CAS No.	61727-69-3
Molecular Formula	C8H14ClNO2
Molecular Weight	191.66 g/mol
Synonyms	N-(tert-Butoxycarbonyl)-L-leucine chloromethyl ketone; Boc-Leu-CH2Cl; L-Leucine Chloromethyl Ketone; (S)-1-Chloro-3-methyl-2-oxopentan-1-amine; Protease Inhibitor (Leucine); N-Boc-L-leucine chloromethyl ketone; TLCK (Leucine variant); Chloromethyl ketone derivative of Boc-Leucine
EINECS	Contact for details

Quality Control

Our Leucine Chloromethyl Ketone is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and chiral analysis to ensure enantiomeric purity. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming compliance with our stringent specifications for research and pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Chiral Purity	≥ 99.0% (S-enantiomer)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.