Description

Benzyloxycarbonylphenylalanyl-Alanine Chloromethyl Ketone is a specialized peptide-derived chloromethyl ketone inhibitor, a crucial tool compound in biochemical and pharmacological research. Its primary value lies in its potent and irreversible inhibition of specific serine proteases, enabling precise mechanistic studies. This compound is essential for researchers and developers in pharmaceutical discovery, proteomics, and enzymology who require high-purity, reliable chemical probes to study enzyme function and develop therapeutic agents.

Application

• Protease Inhibition Research: Acts as an active-site directed, irreversible inhibitor for studying the mechanism and kinetics of serine proteases like chymotrypsin and related enzymes.
• Pharmaceutical Development: Serves as a key intermediate or prototype inhibitor in the design and synthesis of novel therapeutic agents targeting protease-driven diseases.
• Biochemical Assay Development: Used as a standard or control compound in enzymatic assays to validate activity and specificity.
• Proteomics & Cell Biology: Employed to selectively inhibit specific proteolytic pathways in cellular systems, aiding in the mapping of protein function and signaling networks.
• Academic & Industrial R&D: A fundamental research chemical in university laboratories and biotech R&D departments focused on enzyme structure and function.

Basic Information

Product Name	Benzyloxycarbonylphenylalanyl-Alanine Chloromethyl Ketone
CAS No.	60525-17-9
Molecular Formula	C21H23ClN2O5
Molecular Weight	418.87 g/mol
Synonyms	Z-Phe-Ala-CH2Cl; Cbz-Phe-Ala-chloromethyl ketone; Z-Phe-Ala-CMK; Benzyloxycarbonyl-L-phenylalanyl-L-alanine chloromethyl ketone; N-Carbobenzoxy-L-phenylalanyl-L-alanine chloromethyl ketone; Protease Inhibitor Z-Phe-Ala-CMK; Chymotrypsin Inhibitor Z-Phe-Ala-CH2Cl; (S)-1-(((S)-3-(Benzyloxy)-1-chloro-2-oxo-3-phenylpropan-2-yl)amino)-1-oxopropan-2-aminium chloride
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylphenylalanyl-Alanine Chloromethyl Ketone is manufactured under controlled conditions to ensure high chemical purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and structural confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all quality parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.