Description

(25R)-3β-[4-o-(2-o-β-D-Glucopyranosyl-β-D-Glucopyranosyl)-β-D-Galactopyranosyloxy]Spirosta-5-Ene is a high-purity, complex steroidal saponin glycoside of significant interest in advanced pharmaceutical and nutraceutical research. This compound serves as a critical reference standard and a key intermediate for the synthesis of bioactive molecules targeting specific metabolic pathways. It is primarily sought by research institutions and manufacturers in the pharmaceutical, biotechnology, and high-end dietary supplement sectors. Its precise structure makes it invaluable for analytical method development, quality control, and the investigation of structure-activity relationships.

Application

• Pharmaceutical Reference Standard: Used for the identification, assay, and purity testing of steroidal saponin-based drugs and herbal extracts in QC/QA laboratories.
• Active Pharmaceutical Ingredient (API) Intermediate: Serves as a key chiral building block in the multi-step synthesis of novel therapeutic agents.
• Biochemical Research: Employed in in vitro and in vivo studies to explore biological activities such as anti-inflammatory, immunomodulatory, or metabolic effects.
• Nutraceutical Development: Acts as a standardized marker compound for the quality assurance of high-value botanical supplements containing saponins.
• Analytical Method Development: Essential for developing and validating precise HPLC, LC-MS, or NMR methods for complex natural product matrices.
• Academic Research: Used in universities and research institutes for pharmacological, phytochemical, and synthetic chemistry studies.

Basic Information

Item	Details
Product Name	(25R)-3β-[4-o-(2-o-β-D-Glucopyranosyl-β-D-Glucopyranosyl)-β-D-Galactopyranosyloxy]Spirosta-5-Ene
CAS No.	60454-78-6
Molecular Formula	C51H82O23
Molecular Weight	1063.18 g/mol
Synonyms	Diosgenin 3-O-[β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside]; (25R)-Spirost-5-en-3β-yl O-β-D-glucopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-galactopyranoside; Diosgenin triglycoside; Diosgenin 3-O-β-chacotrioside; Steroidal saponin glycoside; Timosaponin B-II (check isomer specificity); Contact for additional trade names.
EINECS	Contact for details

Quality Control

Our (25R)-3β-[4-o-(2-o-β-D-Glucopyranosyl-β-D-Glucopyranosyl)-β-D-Galactopyranosyloxy]Spirosta-5-Ene is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, specific optical rotation, and identity confirmation by IR and NMR, to ensure it meets the stringent requirements for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 95.0%
Specific Optical Rotation	Contact for details
Loss on Drying	≤ 5.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.