Description

4Alpha-Phorbol12-Myristate13-Acetate (CAS NO 6357-44-4) is a potent and specific protein kinase C (PKC) activator widely used in biochemical and pharmacological research. This compound is critical for studying signal transduction pathways, cellular differentiation, and tumor promotion mechanisms. It is an essential tool for researchers in pharmaceutical development, oncology, and immunology, where precise control of PKC activity is required.

Application

• Biomedical Research: As a standard PKC activator for studying enzyme kinetics, signal transduction cascades, and second messenger systems.
• Immunology Studies: Used to stimulate T-cell activation, cytokine production, and oxidative burst in neutrophils for immunological assays.
• Oncology Research: Employed as a tumor promoter in model systems to investigate carcinogenesis and evaluate potential chemopreventive agents.
• Cell Biology: Induces differentiation and modulates proliferation in various cell lines, including HL-60 and other leukemia cell models.
• Drug Discovery: Serves as a key pharmacological tool for high-throughput screening (HTS) to identify novel PKC inhibitors or modulators.
• Inflammation Models: Applied in research to induce inflammatory responses and study the efficacy of anti-inflammatory compounds.

Basic Information

Product Name	4Alpha-Phorbol12-Myristate13-Acetate
CAS No.	6357-44-4
Molecular Formula	C36H56O8
Molecular Weight	616.83 g/mol
Synonyms	PMA; Phorbol 12-Myristate 13-Acetate; TPA; Tetradecanoylphorbol Acetate; 12-O-Tetradecanoylphorbol-13-acetate; NSC 350836; Phorbol myristate acetate; 4α-Phorbol 12-myristate 13-acetate
EINECS	228-768-6

Quality Control

Our 4Alpha-Phorbol12-Myristate13-Acetate is produced under strict quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to comprehensive analytical testing, including identity confirmation by spectroscopic methods and purity assessment by HPLC. A Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Biological Activity	Conforms to reference standard activity in cell-based assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.