Description

Nutlin-3 is a potent and selective small-molecule antagonist of the MDM2-p53 protein-protein interaction, representing a critical tool compound in oncology and molecular biology research. This compound matters because it serves as a chemical probe to reactivate the p53 tumor suppressor pathway in cancer cells with wild-type p53, offering a powerful mechanism for studying apoptosis and cell cycle arrest. Researchers in pharmaceutical R&D, academic institutions, and biotechnology companies need it for target validation, high-throughput screening, and the development of novel cancer therapeutics.

Application

• Oncology Research & Drug Discovery: A key chemical tool for studying the p53 pathway, validating MDM2 as a therapeutic target, and screening for synergistic compounds.
• Apoptosis & Cell Cycle Studies: Used to induce p53-dependent apoptosis and cell cycle arrest (G1 and G2/M phases) in various cancer cell lines for mechanistic studies.
• Preclinical Cancer Models: Employed in in vitro and in vivo models to evaluate the efficacy of p53 pathway reactivation as a therapeutic strategy.
• Biochemical Assay Development: Serves as a reference standard and positive control in fluorescence polarization (FP), surface plasmon resonance (SPR), and other binding assays targeting the MDM2-p53 interaction.
• Chemical Biology & Proteomics: Utilized to investigate protein-protein interaction networks and downstream signaling events following p53 stabilization.
• Combination Therapy Research: Investigated for potential synergistic effects with chemotherapy, radiotherapy, and other targeted agents.

Basic Information

Product Name	Nutlin-3
CAS No.	548472-68-0
Molecular Formula	C30H30Cl2N4O4
Molecular Weight	581.49 g/mol
Synonyms	Nutlin 3; Nutlin-3a; (-)-Nutlin-3; cis-Nutlin-3; 4-[4,5-Bis(4-chlorophenyl)-2-(2-isopropoxy-4-methoxyphenyl)-4,5-dihydro-1H-imidazol-1-yl]carbonyl]piperazin-2-one; MDM2 antagonist Nutlin-3; RG-7112 (related active enantiomer)
EINECS	Contact for details

Quality Control

Our Nutlin-3 is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity determination, 1H/13C NMR and mass spectrometry for structural confirmation, and residual solvent analysis. A detailed Certificate of Analysis (COA) documenting identity, purity, and strength is provided with every shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.