Description

Ape1 Inhibitor Iii is a potent and selective small molecule inhibitor of the apurinic/apyrimidinic endonuclease 1 (APE1/Ref-1) enzyme. This compound is critical for researchers and developers targeting DNA base excision repair (BER) pathways, offering a valuable tool for studying cancer cell resistance mechanisms and developing novel therapeutic strategies. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and biotechnology companies focused on oncology, neurodegenerative diseases, and radiobiology.

Application

• Biomedical Research: A key reagent for investigating the role of APE1 in DNA repair, redox signaling, and its impact on cancer cell survival and chemoresistance.
• Oncology Drug Discovery: Used in high-throughput screening and validation studies to identify and develop combination therapies that sensitize tumors to DNA-damaging agents like alkylators and ionizing radiation.
• Mechanistic Studies: Essential for elucidating the structure-activity relationship (SAR) of APE1 inhibition and its downstream effects on gene expression and apoptosis.
• Preclinical Development: Serves as a reference compound or lead molecule in the development of novel APE1-targeted therapeutics for various cancers.
• Neurodegenerative Disease Research: Applied in models to study oxidative stress and DNA damage in conditions like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease.

Basic Information

Product Name	Ape1 Inhibitor Iii
CAS No.	524708-03-0
Molecular Formula	C₂₀H₂₀N₄O₃
Molecular Weight	364.40 g/mol
Synonyms	7-Nitro-1H-indole-2-carboxylic acid {4-[1-(guanidinohydrazone)ethyl]phenyl}amide; APE1 Inhibitor III; APE1-IN-3; AR03; Ref-1 Inhibitor; APEX1 Inhibitor; (4-{1-[(E)-(diaminomethylidene)hydrazono]ethyl}phenyl)amide of 7-nitro-1H-indole-2-carboxylic acid
EINECS	Contact for details

Quality Control

Our Ape1 Inhibitor Iii is manufactured under strict quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and structural confirmation via NMR and mass spectrometry. A detailed Certificate of Analysis (COA) is provided, confirming compliance with our stringent in-house specifications for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, moisture-free environment. The product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.