Description

H-[15N]Leu-Oh is a stable isotope-labeled analog of the essential amino acid L-Leucine, specifically enriched with Nitrogen-15 at the indicated position. This compound is a critical tool for advanced research requiring precise metabolic and proteomic tracing. It is primarily utilized by scientists and manufacturers in the fields of pharmaceutical development, nutritional studies, and analytical chemistry for applications such as mass spectrometry, NMR spectroscopy, and stable isotope probing.

Application

• Metabolic Pathway Analysis: Used as a tracer in in vivo and in vitro studies to investigate protein synthesis, turnover, and amino acid metabolism.
• Quantitative Proteomics: Serves as a crucial component in Stable Isotope Labeling by Amino acids in Cell culture (SILAC) for accurate protein quantification and biomarker discovery.
• NMR Spectroscopy: Acts as a non-radioactive probe for structural and dynamic studies of proteins and peptides, leveraging the distinct magnetic properties of 15N.
• Pharmaceutical Research: Employed in the development and testing of new drugs, particularly in pharmacokinetic studies to track the fate of drug compounds.
• Nutritional Science: Used to study amino acid requirements, protein metabolism, and the efficacy of nutritional supplements in clinical research.
• Internal Standard for MS: Functions as a high-purity internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) for the absolute quantification of leucine and related metabolites.

Basic Information

Product Name	H-[15N]Leu-Oh
CAS No.	59935-31-8
Molecular Formula	C6H1215NO2
Molecular Weight	132.16 g/mol (for 15N isotopologue)
Synonyms	L-Leucine-15N; (S)-2-Amino-4-methylpentanoic acid-15N; Leucine-15N; 15N-L-Leucine; H-Leu(15N)-OH; (2S)-2-Azanyl-4-methylpentanoic acid-15N; Stable Isotope Labeled L-Leucine; Amino acid-15N tracer
EINECS	Contact for details

Quality Control

Our H-[15N]Leu-Oh is manufactured under strict quality control protocols to ensure exceptional isotopic and chemical purity, essential for sensitive research applications. Each batch is analyzed using advanced techniques including NMR, HPLC, and Mass Spectrometry. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥98 atom % 15N), chemical purity, and specific analytical data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Isotopic Enrichment	≥98 atom % 15N
Assay (HPLC)	≥98.0% (chemical purity)
Optical Rotation	[α]D20: +14.5° to +16.5° (c=2 in 6N HCl)
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.