Description

Cholesterol-3-18O is a stable isotope-labeled analog of cholesterol, where the oxygen atom at the 3-position is replaced with the non-radioactive isotope Oxygen-18 (¹⁸O). This compound is a critical internal standard and tracer in advanced analytical and metabolic research. It is essential for scientists and manufacturers requiring precise quantification and pathway elucidation in lipid biochemistry. Key users include pharmaceutical R&D laboratories, academic research institutions, and diagnostic kit developers focused on cardiovascular and metabolic diseases.

Application

• Quantitative Mass Spectrometry (MS): Serves as a crucial internal standard for the accurate quantification of cholesterol and its metabolites in biological samples via GC-MS or LC-MS.
• Metabolic Pathway Tracing: Used in isotope dilution studies to trace and elucidate cholesterol biosynthesis, absorption, and turnover rates in vivo and in vitro.
• Pharmaceutical Development: Employed in the research and development of lipid-lowering drugs, enabling precise pharmacokinetic and pharmacodynamic studies.
• Clinical Diagnostics: Acts as a reference standard in the calibration and validation of diagnostic assays for cholesterol-related disorders.
• Biochemical Research: Facilitates studies on membrane lipid dynamics, lipoprotein metabolism, and steroid hormone synthesis pathways.
• Quality Control & Method Validation: Used to develop and validate robust analytical methods in regulatory and quality control environments.

Basic Information

Product Name	Cholesterol-3-18O
CAS No.	59613-51-3
Molecular Formula	C₂₇H₄₆¹⁸O
Molecular Weight	388.66 g/mol (for ¹⁸O isotope)
Synonyms	Cholesterol-3-¹⁸O; (3β)-Cholest-5-en-3-ol-18O; 18O-Labeled Cholesterol; Cholesterol (oxygen-18O); 5-Cholesten-3β-ol-18O; Isotope Labeled Cholesterol; Cholesterol Internal Standard (¹⁸O); Stable Isotope-Labeled Cholesterol
EINECS	Contact for details

Quality Control

Our Cholesterol-3-18O is manufactured under strict quality systems to ensure high isotopic and chemical purity, critical for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically >98 atom % ¹⁸O), chemical purity (by HPLC), and identity confirmation (by NMR and MS). We adhere to relevant industry standards for research chemicals, ensuring traceability and reliability for your critical studies.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. The product is light-sensitive and easily oxidized; prolonged exposure to air, light, or elevated temperatures should be avoided to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Isotopic Enrichment (Atom % ¹⁸O)	≥ 98%
Chemical Purity (HPLC)	≥ 98%
Specific Rotation	Contact for details
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.