Description

H-Ile-Cmk·HCl is a chloromethyl ketone derivative of isoleucine, a potent and irreversible inhibitor of serine proteases. This compound is critical for biochemical research and pharmaceutical development, enabling precise study and control of enzymatic activity. It is primarily utilized by researchers in proteomics, medicinal chemistry, and drug discovery for investigating enzyme mechanisms and developing therapeutic agents.

Application

• Protease Inhibition Studies: Acts as a specific, irreversible active-site inhibitor for serine proteases such as trypsin, chymotrypsin, and elastase.
• Biochemical Research: A key tool compound for studying enzyme kinetics, catalytic mechanisms, and protein function in vitro.
• Medicinal Chemistry & Drug Discovery: Serves as a pharmacophore or building block in the design and synthesis of protease-targeted therapeutic candidates.
• Proteomics & Cell Biology: Used to selectively inhibit specific proteolytic pathways to understand their role in cellular processes and disease states.
• Diagnostic Reagent Development: Employed in the formulation of assay kits and diagnostic tools that require controlled protease activity.

Basic Information

Product Name	H-Ile-Cmk·HCl
CAS No.	59079-44-6
Molecular Formula	C10H19ClN2O2·HCl
Molecular Weight	279.19 g/mol
Synonyms	Isoleucine Chloromethyl Ketone Hydrochloride; L-Isoleucyl Chloromethyl Ketone Hydrochloride; H-Ile-CH2Cl·HCl; Isoleucine CMK HCl; N-(tert-Butoxycarbonyl)-L-isoleucyl chloromethyl ketone hydrochloride (related Boc-protected precursor); Protease Inhibitor (Ile-CMK); TLCK analog (Ile variant); Ac-Ile-CH2Cl (related acetylated form)
EINECS	Contact for details

Quality Control

Our H-Ile-Cmk·HCl is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture content. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to ensure traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.