Description

Phorbol 12-Myristate 13-Acetate 4-o-Methyl Ether, 4Beta is a specific, semi-synthetic derivative of phorbol ester, a class of compounds known for their potent biological activity. This compound is a critical research-grade biochemical tool, primarily valued for its role as a highly specific activator of Protein Kinase C (PKC) isoforms. It is essential for researchers and developers in life sciences and pharmaceutical discovery who require precise molecular probes to study cell signaling pathways, inflammation, and tumor promotion mechanisms.

Application

• Biochemical Research: A key tool for activating specific isoforms of Protein Kinase C (PKC) in cell-based and in vitro studies.
• Cell Signaling Pathway Analysis: Used to investigate intracellular signal transduction mechanisms related to proliferation, differentiation, and apoptosis.
• Immunology and Inflammation Studies: Employed to stimulate immune cells like T-cells and macrophages to model inflammatory responses.
• Cancer Research: Utilized in studies exploring the role of PKC in tumor promotion, metastasis, and cell transformation.
• Neuroscience Research: Applied in studies of neurotransmitter release, neuronal plasticity, and neurodegenerative processes where PKC is involved.
• Pharmaceutical Screening: Serves as a reference agonist in high-throughput screening assays to identify novel PKC inhibitors or modulators.

Basic Information

Product Name	Phorbol 12-Myristate 13-Acetate 4-o-Methyl Ether, 4Beta
CAS No.	57716-89-9
Molecular Formula	C37H56O8
Molecular Weight	628.85 g/mol
Synonyms	4-O-Methyl-12-O-tetradecanoylphorbol-13-acetate; 4β-Phorbol 12-myristate 13-acetate 4-methyl ether; 4-O-Methyl PMA; 4-O-Methylphorbol 12-myristate 13-acetate; 4-O-Methyl TPA; 4-O-Methyl-12-O-tetradecanoylphorbol 13-acetate; 4β-PMA methyl ether
EINECS	Contact for details

Quality Control

Our research-grade Phorbol 12-Myristate 13-Acetate 4-o-Methyl Ether, 4Beta is produced under strict quality protocols to ensure high purity, identity, and batch-to-batch consistency, which are critical for reproducible scientific results. Each batch is fully characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and solvent content is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer. The product is light-sensitive and should be handled under appropriate conditions to maintain stability. Allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.