Description

Equilenin-4,16,16-D3 is a deuterated derivative of the natural estrogenic steroid equilenin, specifically labeled with three deuterium atoms at the 4,16, and 16 positions. This isotopic labeling is critical for enhancing analytical precision and metabolic stability in research applications. It serves as a vital internal standard and tracer in mass spectrometry-based assays within pharmaceutical development and clinical research laboratories. This compound is essential for scientists requiring high-purity, isotopically labeled reference materials for quantitative bioanalysis and metabolic studies.

Application

• Internal Standard for LC-MS/MS: Used as a stable isotope-labeled internal standard for the precise quantification of equilenin and related compounds in biological matrices via liquid chromatography-tandem mass spectrometry.
• Metabolic Pathway Tracing: Employed in pharmacokinetic and metabolic stability studies to trace and elucidate the biotransformation pathways of steroid compounds.
• Pharmaceutical Reference Standard: Serves as a certified reference material (CRM) for method development, validation, and quality control in pharmaceutical analysis and regulatory submissions.
• Biomedical Research: Utilized in endocrine and steroid hormone research to investigate receptor binding, enzyme activity, and hormonal signaling without interference from endogenous compounds.
• Isotope Dilution Analysis: Applied in high-accuracy analytical techniques that require compensation for matrix effects and instrument variability.

Basic Information

Product Name	Equilenin-4,16,16-D3
CAS No.	56588-54-6
Molecular Formula	C18H15D3O2
Molecular Weight	273.37 g/mol
Synonyms	Equilenin-d3; 3-Hydroxyestra-1,3,5(10),6,8-pentaen-17-one-4,16,16-d3; 1,3,5(10),6,8-Estratetraene-3,17-diol-17-one-4,16,16-d3; 4,16,16-Trideuteroequilenin; [4,16,16-2H3]-Equilenin; Deuterated Equilenin; Isotopically Labeled Equilenin
EINECS	Contact for details

Quality Control

Our Equilenin-4,16,16-D3 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for chemical purity and isotopic enrichment, NMR for structural confirmation, and mass spectrometry for identity and isotopic distribution verification. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes batch-specific data on purity, assay, and deuterium content. Our quality commitment ensures the material is suitable for use as a high-grade reference standard in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at 2-8°C. Keep the container sealed to prevent moisture absorption and contamination.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D3)	≥ 98.0 atom % D
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.