Description

21-Hydroxy-20-Oxo-5β-Pregnan-3α-Yl β-D-Glucopyranosiduronic Acid CAS NO 56162-36-8 is a high-purity steroid glucuronide derivative, a critical reference standard and intermediate in advanced pharmaceutical research and development. Its value lies in providing a definitive benchmark for the analysis, quantification, and metabolic study of related steroid hormones and their conjugates. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require precise analytical standards for method validation, quality control, and metabolic pathway investigation.

Application

• Pharmaceutical Reference Standard: Serves as a certified primary standard for the calibration of analytical instruments (HPLC, LC-MS) and the validation of assay methods for steroid metabolites.
• Metabolic Pathway Research: Used as a key intermediate or marker in studying the glucuronidation pathways and pharmacokinetics of pregnane-based steroid hormones.
• Diagnostic Reagent Development: Employed in the synthesis and formulation of critical components for immunoassays and clinical diagnostic kits targeting specific steroid profiles.
• Biochemical Research: Acts as a substrate or inhibitor in enzymatic studies to understand the function of glucuronosyltransferases (UGTs) and other metabolic enzymes.
• Impurity Profiling: Utilized as an identified impurity standard to monitor and control the quality of bulk active pharmaceutical ingredients (APIs) in steroid manufacturing.

Basic Information

Product Name	21-Hydroxy-20-Oxo-5β-Pregnan-3α-Yl β-D-Glucopyranosiduronic Acid
CAS No.	56162-36-8
Molecular Formula	C27H40O9
Molecular Weight	508.60 g/mol
Synonyms	21-Hydroxy-20-oxopregnan-3α-yl β-D-glucopyranosiduronic acid; 3α,21-Dihydroxy-5β-pregnan-20-one 3-glucuronide; 5β-Pregnan-3α,21-diol-20-one 3-glucuronide; Tetrahydrocorticosterone 3α-glucuronide; THB 3α-Glucuronide; 3α-Glucuronide of 5β-Pregnan-3α,21-diol-20-one; 21-Hydroxy-20-oxo-5β-pregnan-3α-yl β-D-glucuronide
EINECS	Contact for details

Quality Control

Every batch of 21-Hydroxy-20-Oxo-5β-Pregnan-3α-Yl β-D-Glucopyranosiduronic Acid is manufactured and tested under strict quality management systems. We provide comprehensive analytical documentation to support its use as a reference material. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Related Substances (HPLC)	Individual impurity ≤ 0.5%; Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.