Description

L-Threonine-13C4 is a stable isotope-labeled analog of the essential amino acid L-Threonine, where all four carbon atoms are replaced with the carbon-13 (13C) isotope. This high-purity labeled compound is critical for applications requiring precise internal standards and metabolic pathway tracing. It is primarily utilized by research institutions, pharmaceutical companies, and diagnostic manufacturers engaged in advanced quantitative mass spectrometry, metabolic flux analysis, and clinical research.

Application

• Internal Standard for Quantitative Analysis: Used as a certified reference material in LC-MS/MS and GC-MS for the accurate quantification of endogenous L-Threonine in complex biological matrices like plasma, urine, and tissue samples.
• Metabolic Research & Fluxomics: Serves as a crucial tracer in 13C metabolic flux analysis (MFA) to study amino acid metabolism, protein synthesis rates, and cellular metabolic networks in vivo and in vitro.
• Pharmaceutical Development: Employed in pharmacokinetic (PK) and pharmacodynamic (PD) studies for drug candidates that interact with amino acid pathways, enabling precise tracking of drug effects.
• Clinical Diagnostics: Essential for developing and calibrating high-sensitivity diagnostic assays for inborn errors of metabolism (IEMs) related to threonine catabolism.
• Nutritional Science Research: Used to investigate protein metabolism, amino acid requirements, and the metabolic fate of dietary threonine in animal and human studies.
• Biotechnology & Fermentation Monitoring: Applied in process optimization for microbial fermentation, allowing for detailed analysis of precursor incorporation and pathway efficiency.

Basic Information

Product Name	L-Threonine-13C4
CAS No.	55443-53-3
Molecular Formula	13C4H9NO3
Molecular Weight	123.08 (based on 13C)
Synonyms	(2S,3R)-2-Amino-3-hydroxy(13C4)butanoic acid; L-13C4-Threonine; (2S,3R)-2-Azaniumyl-3-hydroxy(13C4)butanoate; Threonine-13C4; U-13C4-L-Threonine; Uniformly Labeled 13C4-L-Threonine; L-Threonine (13C4)
EINECS	Contact for details

Quality Control

Our L-Threonine-13C4 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research and diagnostic applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥99 atom % 13C), chemical purity (by HPLC), chiral purity, and spectroscopic confirmation. We adhere to relevant guidelines for stable isotope-labeled compounds to ensure batch-to-batch consistency and reliability as a quantitative standard.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive). For long-term storage, keep the container in a cool, dry place with desiccant to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥99 atom % 13C
Isotopic Purity	≥99% 13C4
Optical Rotation	-28.0° to -30.0° (c=1 in H2O)
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.