Description

Leupeptin Hemisulfate Monohydrate is a potent and reversible protease inhibitor, specifically targeting serine, cysteine, and threonine peptidases. This compound is a critical research tool for studying intracellular proteolytic pathways, including apoptosis and signal transduction. It is essential for scientists in biochemical research, pharmaceutical development, and cell biology, where precise control of protease activity is required.

Application

• Biochemical Research: Used as a standard inhibitor to study the mechanisms of proteases like trypsin, plasmin, and cathepsin B in vitro.
• Cell Culture & Apoptosis Studies: Added to cell culture media to inhibit calpain and other proteases, preventing artifactual protein degradation and studying programmed cell death.
• Protein Purification & Analysis: Incorporated into lysis and extraction buffers to preserve protein integrity by inhibiting endogenous proteases during sample preparation.
• Pharmaceutical Screening: Serves as a reference compound in drug discovery programs targeting protease-related diseases.
• Enzyme Kinetics: Employed to determine kinetic parameters and inhibition constants (Ki) for various peptidases.
• Diagnostic Reagent Manufacturing: Used in the formulation of specific diagnostic kits where protease activity must be controlled.

Basic Information

Product Name	Leupeptin Hemisulfate Monohydrate
CAS No.	55123-66-5
Molecular Formula	C₂₀H₃₈N₆O₄ · ½H₂SO₄ · H₂O
Molecular Weight	548.66 g/mol (as monohydrate hemisulfate)
Synonyms	Leupeptin sulfate salt; Leupeptin hemisulfate; Acetyl-Leu-Leu-Arg-al hemisulfate; N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate; Protease Inhibitor Leupeptin; (2S)-1-[(2S)-2-Acetamido-4-methylpentanoyl]-N-[(3S,8S)-3-amino-8-methyl-1-oxo-4,7,10-triazadodec-11-en-8-yl]-4-methylpentanamide hemisulfate; NSC 332500
EINECS	Contact for details

Quality Control

Our Leupeptin Hemisulfate Monohydrate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent biological activity. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications for identity, purity, and potency. We support research and GMP-grade requirements, with documentation available for audit purposes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	3.0% - 6.0%
Solubility	Soluble in water, DMSO
Biological Activity	Tested for protease inhibition

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.