Description

L-Leucine Chloromethyl Ketone Hydrochloride is a specialized biochemical reagent belonging to the class of amino acid-derived protease inhibitors. This compound is a critical tool for researchers in enzymology and medicinal chemistry, serving as a potent and irreversible active-site directed inhibitor. It is primarily utilized in the study of serine and cysteine proteases, enabling the investigation of enzyme mechanisms and the development of therapeutic agents. Key industries that rely on this high-purity intermediate include pharmaceutical R&D, biotechnology, and academic research institutions.

Application

• Protease Inhibition Studies: Acts as an active-site directed, irreversible inhibitor for characterizing serine and cysteine protease activity and kinetics.
• Medicinal Chemistry & Drug Discovery: Serves as a key building block or pharmacophore in the design and synthesis of novel protease-targeting therapeutic candidates.
• Biochemical Research: Used as a specific tool compound to probe enzyme function, substrate specificity, and catalytic mechanisms in complex biological systems.
• Peptide Synthesis: Employed as a specialized derivative in the synthesis of peptide-based inhibitors or affinity labels.
• Diagnostic Reagent Development: Utilized in the formulation of enzyme assay kits and diagnostic tools for detecting specific protease activities.

Basic Information

Product Name	L-Leucine Chloromethyl Ketone Hydrochloride
CAS No.	54518-92-2
Molecular Formula	C8H15Cl2NO2
Molecular Weight	228.12 g/mol
Synonyms	N-(tert-Butoxycarbonyl)-L-leucine chloromethyl ketone hydrochloride; L-Leucyl chloromethyl ketone hydrochloride; Z-Leu-CH2Cl hydrochloride; Boc-Leu-CH2Cl·HCl; (S)-2-((tert-Butoxycarbonyl)amino)-4-methyl-1-chloropentan-1-one hydrochloride; Protease inhibitor (leucine-derived); TLCK analog (leucine variant)
EINECS	Contact for details

Quality Control

Our L-Leucine Chloromethyl Ketone Hydrochloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis to confirm stereochemical integrity, and identity confirmation via IR and NMR spectroscopy. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.