Description

Cytosine-2-14C is a carbon-14 labeled isotopologue of the fundamental pyrimidine nucleobase, cytosine, where the radioactive isotope is specifically incorporated at the 2-position of the heterocyclic ring. This compound is essential for conducting precise metabolic and pharmacokinetic tracer studies, enabling researchers to track the incorporation, distribution, and fate of cytosine and its derivatives within biological systems. It is a critical reagent for scientists in pharmaceutical R&D, biochemistry, and molecular biology who require high-specific-activity radiolabeled compounds for advanced research.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used as a radiolabeled tracer to investigate the absorption, distribution, metabolism, and excretion (ADME) profiles of cytosine-based pharmaceuticals and nucleoside analogs.
• Nucleic Acid Metabolism Research: Employed to study the biosynthesis, turnover, and salvage pathways of DNA and RNA, providing insights into cellular replication and gene expression mechanisms.
• Biochemical Pathway Tracing: Facilitates the elucidation of complex metabolic pathways involving pyrimidines, helping to map out enzymatic reactions and identify potential drug targets.
• Autoradiography and Imaging: Serves as a probe in autoradiographic techniques to visualize the spatial localization of cytosine incorporation in tissues, cells, or chromatographic plates.
• Environmental and Agricultural Fate Studies: Applied in research to track the environmental degradation and plant uptake of cytosine-related compounds, such as certain fungicides or plant growth regulators.
• Reference Standard for Analytical Methods: Acts as an internal standard or calibrant in advanced analytical techniques like Liquid Chromatography-Mass Spectrometry (LC-MS) for the quantification of cytosine and its metabolites.

Basic Information

Product Name	Cytosine-2-14C
CAS No.	52659-21-9
Molecular Formula	C4H514CN3O
Molecular Weight	112.10 g/mol (for unlabeled base)*
Synonyms	2-14C-Cytosine; 4-Amino-2(14C)-hydroxypyrimidine; 6-Amino-2(14C)-hydroxypyrimidine; Cytosine-2-14C; [2-14C]Cytosine; 2-14C-Labelled Cytosine; Cytosine (2-14C)
EINECS	Contact for details

*Molecular weight is approximate and varies with specific activity.

Quality Control

Every batch of Cytosine-2-14C is manufactured and analyzed under strict quality systems. We provide comprehensive analytical documentation to ensure traceability and reliability for your research. Certificates of Analysis (COA) are supplied with each shipment, detailing critical parameters such as radiochemical purity, specific activity, chemical purity, and solvent content. Our quality commitment aligns with the stringent requirements for radiolabeled compounds used in GLP-compliant and preclinical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material under an inert atmosphere. Follow all applicable radiation safety guidelines and local regulations for the storage of radioactive materials.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC, MS)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Specific Activity	50-60 mCi/mmol (Contact for specific value)
Solvent (if supplied in solution)	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.