Description

Z-Leu-Chloromethylketone is a specialized, irreversible protease inhibitor belonging to the chloromethyl ketone class of synthetic peptides. This compound is a critical research tool for the study of serine and cysteine protease mechanisms, acting as an active-site-directed affinity label. It is primarily utilized by researchers and process development scientists in the pharmaceutical and life sciences sectors for biochemical research, enzyme characterization, and inhibitor design.

Application

• Biochemical Research: As a potent and irreversible inhibitor for mechanistic studies of serine proteases like trypsin, chymotrypsin, and related enzymes.
• Protease Inhibition Studies: Used to elucidate enzyme kinetics, active site architecture, and catalytic pathways in academic and industrial R&D.
• Pharmaceutical Development: Serves as a key intermediate or reference standard in the development of therapeutic protease inhibitors.
• Cell Biology & Apoptosis Research: Applied in studies investigating the role of specific proteases in cellular signaling and programmed cell death pathways.
• Peptide Chemistry: Used as a building block or modifying agent in the synthesis of more complex peptide-based inhibitors and probes.

Basic Information

Product Name	Z-Leu-Chloromethylketone
CAS No.	52467-54-6
Molecular Formula	C15H19ClN2O4
Molecular Weight	326.78 g/mol
Synonyms	Z-Leu-CMK; N-Carbobenzoxy-L-leucyl chloromethyl ketone; Benzyloxycarbonyl-L-leucyl chloromethyl ketone; L-Leucine, N-[(phenylmethoxy)carbonyl]-, 1-(chloromethyl)-2-oxoethyl ester; (S)-1-Chloro-3-((((benzyloxy)carbonyl)amino)-4-methyl-1-oxopentan-2-yl)aminopropan-2-one; Z-L-Leu-CMK; CBZ-Leu-CMK; Protease Inhibitor Z-Leu-CMK
EINECS	Contact for details

Quality Control

Our Z-Leu-Chloromethylketone is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and related substances is provided with every shipment to support your research and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in alcohols

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.