Description

5Alpha-Androstan-17Beta-Ol-3-One-2,2,4,4-D4 is a high-purity, deuterium-labeled analog of dihydrotestosterone (DHT), a key androgen hormone. This stable isotope-labeled compound is essential for precise analytical quantification and metabolic pathway research, offering superior accuracy in mass spectrometry-based assays. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and analytical service providers for use as an internal standard in bioanalytical method development, pharmacokinetic studies, and endocrinology research.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of endogenous DHT and related steroids in complex biological matrices like serum, plasma, and tissue homogenates.
• Metabolic Tracing Studies: Used in stable isotope dilution assays to investigate androgen metabolism, biosynthesis, and degradation pathways in research settings.
• Pharmaceutical Development: Supports bioanalytical method validation for drug candidates targeting androgen receptors or steroidogenic enzymes.
• Clinical Research & Diagnostics: Enables precise measurement of androgen levels in studies related to endocrine disorders, such as androgen insensitivity, PCOS, and prostate health.
• Reference Material: Serves as a certified reference material (CRM) for calibrating analytical instruments and ensuring data integrity in regulated laboratories.
• Doping Control Analysis: Employed in sports anti-doping laboratories for the definitive identification and quantification of prohibited anabolic androgenic steroids.

Basic Information

Product Name	5Alpha-Androstan-17Beta-Ol-3-One-2,2,4,4-D4
CAS No.	5295-66-9
Molecular Formula	C19H26D4O2
Molecular Weight	294.47 g/mol
Synonyms	Dihydrotestosterone-d4; DHT-d4; 5α-Dihydrotestosterone-2,2,4,4-d4; 17β-Hydroxy-5α-androstan-3-one-2,2,4,4-d4; Androstanolone-d4; 5α-Androstan-17β-ol-3-one-2,2,4,4-d4; NSC 13767-d4; Stanolone-d4
EINECS	Contact for details

Quality Control

Our deuterated standards are manufactured under strict quality systems to ensure the highest isotopic purity and chemical integrity. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and high-resolution mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing isotopic enrichment (typically ≥98 atom % D), chemical purity (≥95% by HPLC), and analytical traceability. We support compliance with ISO 17034 and GMP guidelines for reference material producers.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.