Description

Esculin is a naturally occurring coumarin glycoside, specifically the 6-β-glucoside of esculetin. This compound is valued for its role as a key biochemical marker and analytical standard in various research and quality control applications. It is primarily sought by professionals in the pharmaceutical, nutraceutical, and analytical chemistry sectors for its utility in enzymatic assays, fluorescence studies, and as a reference material for plant extract analysis.

Application

• Biochemical Research: Used as a standard substrate for the enzyme β-glucosidase in enzymatic activity assays and inhibition studies.
• Analytical Chemistry: Serves as a high-purity reference standard in HPLC, LC-MS, and TLC for the identification and quantification of coumarins in complex matrices like plant extracts and herbal products.
• Fluorescence Applications: Employed in laboratory settings as a fluorescent probe or marker due to its intrinsic fluorescent properties under UV light.
• Pharmaceutical Development: Utilized in research exploring the biological activities of coumarin derivatives, including potential antioxidant and anti-inflammatory effects.
• Quality Control: Acts as a critical purity indicator in the standardization of raw materials derived from horse chestnut (*Aesculus hippocastanum*) and other botanical sources.
• Microbiology: Applied in selective media as a differential agent for the identification of certain bacterial species, notably in conjunction with ferric citrate.

Basic Information

Item	Detail
Product Name	Esculin
CAS No.	531-75-9
Molecular Formula	C15H16O9
Molecular Weight	340.28 g/mol
Synonyms	Esculoside; Aesculin; Esculin hydrate; 6,7-Dihydroxycoumarin-6-glucoside; 6-Glucosyloxy-7-hydroxycoumarin; Polychrome; β-D-Glucopyranoside, 6,7-dihydroxy-2H-1-benzopyran-2-one; (6-Hydroxy-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one)
EINECS	208-517-5

Quality Control

Our Esculin is produced and tested under a rigorous quality management system. Each batch is analyzed to ensure high purity and consistency, with specifications typically meeting or exceeding standard laboratory reagent grades. A comprehensive Certificate of Analysis (COA) detailing identity, purity (by HPLC), and related substance tests is provided with every shipment to guarantee traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Related Substances (HPLC)	Individual impurity ≤ 0.5%; Total impurities ≤ 1.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.