Description

Biotinyl-Tyr-Val-Ala-Asp-Chloromethylketone CAS NO 402474-75-3 is a biotinylated, irreversible caspase inhibitor designed for advanced biochemical research. This compound is a critical tool for studying apoptotic pathways, enabling the specific labeling and isolation of active caspase enzymes. It is primarily utilized by researchers and scientists in the fields of pharmaceutical development, oncology research, and molecular biology to investigate programmed cell death mechanisms and screen for potential therapeutic agents.

Application

• Biochemical Research: As an active-site directed probe for the study of caspase activation and regulation in apoptotic pathways.
• Proteomics & Target Identification: Used in affinity purification and pull-down assays to isolate and identify caspase complexes from cell lysates.
• Drug Discovery & Screening: Serves as a key reagent in high-throughput screening assays to identify novel caspase inhibitors for therapeutic development.
• Oncology Research: Employed to investigate the role of caspases in cancer cell death and resistance mechanisms.
• Enzyme Kinetics: Utilized to determine kinetic parameters and inhibition constants (Ki) for caspase enzymes.
• Diagnostic Development: Potential use in the development of diagnostic tools for detecting caspase activity in biological samples.

Basic Information

Product Name	Biotinyl-Tyr-Val-Ala-Asp-Chloromethylketone
CAS No.	402474-75-3
Molecular Formula	C39H56N8O11S
Molecular Weight	844.98 g/mol
Synonyms	Biotin-YVAD-CMK; Biotin-Asp(OMe)-CH2Cl; Biotinylated Caspase-1 Inhibitor; Ac-YVAD-CMK, Biotin conjugate; Biotin-Tyr-Val-Ala-Asp-chloromethyl ketone; Biotin-Aspartyl(OMe)-fluoromethyl ketone analog; Biotin-DEVD-CHO related inhibitor; Biotin-conjugated peptide chloromethylketone
EINECS	Contact for details

Quality Control

Our Biotinyl-Tyr-Val-Ala-Asp-Chloromethylketone is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and structural confirmation by mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.